ABSTRACT DNA replication stress is one of the primary causes of genome instability. In
response to replication stress, cells can employ replication restart mechanisms that rely on …

CRISPR/Cas9-mediated gene editing has great potential utility for treating genetic diseases.
However, its therapeutic applications are limited by unintended genomic alterations arising …

Abstract Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)‐based
“active genetic” elements developed in 2015 bypassed the fundamental rules of traditional …

Abstract CRISPR-interference (CRISPRi), a highly effective method for silencing genes in
mammalian cells, employs an enzymatically dead form of Cas9 (dCas9) complexed with one …

The CRISPR-Cas nickase system for genome editing has attracted considerable attention
owing to its safety, efficiency, and versatility. Although alternative effectors to Cas9 have the …

DNA double-strand breaks (DSBs) represent one of the most severe threats to genomic
integrity, demanding intricate repair mechanisms within eukaryotic cells. A diverse array of …

Mutations in genomic sequences exhibit a strong correlation with various pathological
processes of cancers [1]. Currently, the next-generation sequencing technique [2] and …

The paired nickases approach, which utilizes clustered regularly interspaced short
palindromic repeats (CRISPR)-CRISPR-associated proteins (Cas) nickase and dual guide …

Simple Summary Many insects are categorized as agricultural pests due to their ability to
transmit diseases and damage crops, which results in significant economic losses. Scientists …

Gene knock-in can be defined as the introduction of precisely determined modifications,
insertions, or replacements to the genome, which enables the generation of reporter cells …