Biocatalysis has undergone revolutionary progress in the past century. Benefited by the
integration of multidisciplinary technologies, natural enzymatic reactions are constantly …

Allosteric regulation plays an important role in many biological processes, such as signal
transduction, transcriptional regulation, and metabolism. Allostery is rooted in the …

Rational protein engineering requires a holistic understanding of protein function. Here, we
apply deep learning to unlabeled amino-acid sequences to distill the fundamental features …

Bacteria-based tumor therapy has recently attracted wide attentions due to its unique
capability in targeting tumors and preferentially colonizing the core area of the tumor …

Cellular processes are carried out by many genes, and their study and optimization requires
multiple levers by which they can be independently controlled. The most common method is …

Recent advances in CRISPR based biotechnologies have greatly expanded our capabilities
to repurpose CRISPR for the development of biomolecular sensors for diagnosing diseases …

In phenylketonuria (PKU) patients, a genetic defect in the enzyme phenylalanine
hydroxylase (PAH) leads to elevated systemic phenylalanine (Phe), which can result in …

A general method to generate biosensors for user-defined molecules could provide
detection tools for a wide range of biological applications. Here, we describe an approach …

Multiplex assays of variant effect (MAVEs), such as deep mutational scans and massively
parallel reporter assays, test thousands of sequence variants in a single experiment. Despite …

Dynamic regulation is a promising strategy for fine-tuning metabolic fluxes in microbial cell
factories. However, few of these synthetic regulatory systems have been developed for …