Structural biologists have traditionally approached cellular complexity in a reductionist
manner in which the cellular molecular components are fractionated and purified before …

Serving as the basis of cell life, interactions between nucleic acids and proteins play
essential roles in fundamental cellular processes. Aptamers are unique single-stranded …

In situ cryo electron tomography of cryo focused ion beam milled samples has emerged in
recent years as a powerful technique for structural studies of macromolecular complexes in …

Single-particle cryo-electron tomography is an emerging technique capable of determining
the structure of proteins imaged within the native context of cells at molecular resolution …

Coronavirus replication is associated with the remodeling of cellular membranes, resulting
in the formation of double-membrane vesicles (DMVs). A DMV-spanning pore was identified …

Cryo-focused ion beam milling of frozen-hydrated cells and subsequent cryo-electron
tomography (cryo-ET) has enabled the structural elucidation of macromolecular complexes …

Structural biology studies inside cells and tissues require methods to thin vitrified specimens
to electron transparency. Until now, focused ion beams based on gallium have been used …

Cryogenic electron microscopy (cryo-EM) can reveal the molecular details of biological
processes in their native, cellular environment at atomic resolution. However, few cells are …

Lamella micromachining by focused ion beam milling at cryogenic temperature (cryo-FIB)
has matured into a preparation method widely used for cellular cryo-electron tomography …

Recent advances have made cryogenic (cryo) electron microscopy a key technique to
achieve near-atomic-resolution structures of biochemically isolated macromolecular …