Current progress in expansion microscopy: chemical strategies and applications

G Wen, V Leen, T Rohand, M Sauer… - Chemical …, 2023 - ACS Publications
Expansion microscopy (ExM) is a newly developed super-resolution technique, allowing
visualization of biological targets at nanoscale resolution on conventional fluorescence …

Expansion microscopy: A chemical approach for super-resolution microscopy

Y Zhuang, X Shi - Current opinion in structural biology, 2023 - Elsevier
Super-resolution microscopy is a series of imaging techniques that bypass the diffraction
limit of resolution. Since the 1990s, optical approaches, such as single-molecular …

In situ architecture of the ciliary base reveals the stepwise assembly of intraflagellar transport trains

H Van den Hoek, N Klena, MA Jordan, G Alvarez Viar… - Science, 2022 - science.org
The cilium is an antenna-like organelle that performs numerous cellular functions, including
motility, sensing, and signaling. The base of the cilium contains a selective barrier that …

Multi-color live-cell STED nanoscopy of mitochondria with a gentle inner membrane stain

T Liu, T Stephan, P Chen… - Proceedings of the …, 2022 - National Acad Sciences
Capturing mitochondria's intricate and dynamic structure poses a daunting challenge for
optical nanoscopy. Different labeling strategies have been demonstrated for live-cell …

Expanded vacuum-stable gels for multiplexed high-resolution spatial histopathology

Y Bai, B Zhu, JP Oliveria, BJ Cannon… - Nature …, 2023 - nature.com
Cellular organization and functions encompass multiple scales in vivo. Emerging high-plex
imaging technologies are limited in resolving subcellular biomolecular features. Expansion …

iU-ExM: nanoscopy of organelles and tissues with iterative ultrastructure expansion microscopy

V Louvel, R Haase, O Mercey, MH Laporte… - Nature …, 2023 - nature.com
Expansion microscopy (ExM) is a highly effective technique for super-resolution
fluorescence microscopy that enables imaging of biological samples beyond the diffraction …

Mitochondrial structure, dynamics, and physiology: light microscopy to disentangle the network

JC Landoni, T Kleele, J Winter, W Stepp… - Annual Review of …, 2024 - annualreviews.org
Mitochondria serve as energetic and signaling hubs of the cell: This function results from the
complex interplay between their structure, function, dynamics, interactions, and molecular …

Far-field super-resolution chemical microscopy

M Tang, Y Han, D Jia, Q Yang, JX Cheng - Light: Science & …, 2023 - nature.com
Far-field chemical microscopy providing molecular electronic or vibrational fingerprint
information opens a new window for the study of three-dimensional biological, material, and …

Cellularization across eukaryotes: Conserved mechanisms and novel strategies

B McCartney, O Dudin - Current Opinion in Cell Biology, 2023 - Elsevier
Many eukaryotes form multinucleated cells during their development. Some cells persist as
such during their lifetime, others choose to cleave each nucleus individually using a …

Ultrastructure expansion microscopy reveals the cellular architecture of budding and fission yeast

K Hinterndorfer, MH Laporte, F Mikus… - Journal of Cell …, 2022 - journals.biologists.com
The budding and fission yeasts Saccharomyces cerevisiae and Schizosaccharomyces
pombe have served as invaluable model organisms to study conserved fundamental cellular …