Improving cell and gene therapy safety and performance using next-generation Nanoplasmid vectors

JA Williams, PA Paez - Molecular Therapy-Nucleic Acids, 2023 - cell.com
The cell and gene therapy industry has employed the same plasmid technology for decades
in vaccination, cell and gene therapy, and as a raw material in viral vector and RNA …

Lactococcus lactis as an Interleukin Delivery System for Prophylaxis and Treatment of Inflammatory and Autoimmune Diseases

GM Campos, MF Américo, A dos Santos Freitas… - Probiotics and …, 2024 - Springer
Target delivery of therapeutic agents with anti-inflammatory properties using probiotics as
delivery and recombinant protein expression vehicles is a promising approach for the …

Complementation of arginine auxotrophy for genetic transformation of Coxiella burnetii by use of a defined axenic medium

KM Sandoz, PA Beare, DC Cockrell… - Applied and …, 2016 - Am Soc Microbiol
Host cell-free (axenic) culture of Coxiella burnetii in acidified citrate cysteine medium-2
(ACCM-2) has provided important opportunities for investigating the biology of this naturally …

Efficient long fragment editing technique enables large-scale and scarless bacterial genome engineering

C Huang, L Guo, J Wang, N Wang, YX Huo - Applied Microbiology and …, 2020 - Springer
Bacteria are versatile living systems that enhance our understanding of nature and enable
biosynthesis of valuable chemicals. Long fragment editing techniques are of great …

Multiplex genetic engineering exploiting pyrimidine salvage pathway-based endogenous counterselectable markers

L Birštonas, A Dallemulle, MS López-Berges… - Mbio, 2020 - Am Soc Microbiol
Selectable markers are indispensable for genetic engineering, yet their number and variety
are limited. Most selection procedures for prototrophic cells rely on the introduction of …

Tailor-made fibroblast-specific and antibiotic-free interleukin 12 plasmid for gene electrotransfer-mediated cancer immunotherapy

U Kamensek, N Tesic, G Sersa, S Kos, M Cemazar - Plasmid, 2017 - Elsevier
Electrotransfer mediated delivery of interleukin-12 (IL-12) gene, encoded on a plasmid
vector, has already been demonstrated to have a potent antitumor efficacy and great …

A Novel Nonantibiotic, lgt-Based Selection System for Stable Maintenance of Expression Vectors in Escherichia coli and Vibrio cholerae

M Terrinoni, SL Nordqvist, S Källgård… - Applied and …, 2018 - Am Soc Microbiol
Antibiotic selection for the maintenance of expression plasmids is discouraged in the
production of recombinant proteins for pharmaceutical or other human uses due to the risks …

Multiplex genetic engineering exploiting pyrimidine salvage pathway-based self-encoded selectable markers

L Birštonas, A Dallemulle, MS López-Berges… - bioRxiv, 2020 - biorxiv.org
Selectable markers are indispensable for genetic engineering, yet their number and variety
is limited. Most selection procedures for prototrophic cells rely on the introduction of …