Recognition of molecular diversity of cell surface proteomes in disease is essential for the
development of targeted therapies. Progress in targeted therapeutics requires establishing …

Peptide aptamers are combinatorial protein reagents that bind to target proteins with a high
specificity and a strong affinity. By so doing, they can modulate the function of their cognate …

Recent studies indicate that the DNA recognition domain of transcription activator-like (TAL)
effectors can be combined with the nuclease domain of FokI restriction enzyme to produce …

Galectin-3 is a multifunctional oncogenic protein found in the nucleus and cytoplasm and
also the extracellular milieu. Although recent studies demonstrated an anti-apoptotic activity …

This unit presents protocols designed to detect interacting proteins. Using yeast as a “test
tube” and transcriptional activation of a reporter system, interacting proteins can be …

Certain types of human papillomaviruses (HPVs) are closely linked to the development of
human cancers. Herein, it is shown that intracellular targeting of the HPV16 E6 oncoprotein …

To study potential roles of plasma membrane-associated extracellular cathepsin B in tumor
cell invasion and metastasis, we used the yeast two-hybrid system to screen for proteins that …

Background Maps depicting binary interactions between proteins can be powerful starting
points for understanding biological systems. A proven technology for generating such maps …

The transcription factor signal transducer and activator of transcription (Stat) 3 is activated
through the interleukin-6 family of cytokines and by binding of growth factors to the …

RNA aptamers selected against proteins can be used to modulate specific protein function.
Expression of such reagents in cells and whole organisms could provide a means of …