Synthetic DNA synthesis and assembly: putting the synthetic in synthetic biology
RA Hughes, AD Ellington - Cold Spring Harbor …, 2017 - cshperspectives.cshlp.org
The chemical synthesis of DNA oligonucleotides and their assembly into synthons, genes,
circuits, and even entire genomes by gene synthesis methods has become an enabling …
circuits, and even entire genomes by gene synthesis methods has become an enabling …
Bricks and blueprints: methods and standards for DNA assembly
DNA assembly is a key part of constructing gene expression systems and even whole
chromosomes. In the past decade, a plethora of powerful new DNA assembly methods …
chromosomes. In the past decade, a plethora of powerful new DNA assembly methods …
Synthetic biology in mammalian cells: next generation research tools and therapeutics
F Lienert, JJ Lohmueller, A Garg… - Nature reviews Molecular …, 2014 - nature.com
Recent progress in DNA manipulation and gene circuit engineering has greatly improved
our ability to programme and probe mammalian cell behaviour. These advances have led to …
our ability to programme and probe mammalian cell behaviour. These advances have led to …
A roadmap for research on crassulacean acid metabolism (CAM) to enhance sustainable food and bioenergy production in a hotter, drier world
Crassulacean acid metabolism (CAM) is a specialized mode of photosynthesis that features
nocturnal CO 2 uptake, facilitates increased water‐use efficiency (WUE), and enables CAM …
nocturnal CO 2 uptake, facilitates increased water‐use efficiency (WUE), and enables CAM …
Integrating biological redesign: where synthetic biology came from and where it needs to go
Synthetic biology seeks to extend approaches from engineering and computation to
redesign of biology, with goals such as generating new chemicals, improving human health …
redesign of biology, with goals such as generating new chemicals, improving human health …
A multi-landing pad DNA integration platform for mammalian cell engineering
L Gaidukov, L Wroblewska, B Teague… - Nucleic acids …, 2018 - academic.oup.com
Engineering mammalian cell lines that stably express many transgenes requires the precise
insertion of large amounts of heterologous DNA into well-characterized genomic loci, but …
insertion of large amounts of heterologous DNA into well-characterized genomic loci, but …
Efficient search, mapping, and optimization of multi‐protein genetic systems in diverse bacteria
I Farasat, M Kushwaha, J Collens… - Molecular systems …, 2014 - embopress.org
Developing predictive models of multi‐protein genetic systems to understand and optimize
their behavior remains a combinatorial challenge, particularly when measurement …
their behavior remains a combinatorial challenge, particularly when measurement …
Multi-kilobase homozygous targeted gene replacement in human induced pluripotent stem cells
Sequence-specific nucleases such as TALEN and the CRISPR/Cas9 system have so far
been used to disrupt, correct or insert transgenes at precise locations in mammalian …
been used to disrupt, correct or insert transgenes at precise locations in mammalian …
Genetically engineering self-organization of human pluripotent stem cells into a liver bud-like tissue using Gata6
Human induced pluripotent stem cells (hiPSCs) have potential for personalized and
regenerative medicine. While most of the methods using these cells have focused on …
regenerative medicine. While most of the methods using these cells have focused on …
High-throughput 5′ UTR engineering for enhanced protein production in non-viral gene therapies
Despite significant clinical progress in cell and gene therapies, maximizing protein
expression in order to enhance potency remains a major technical challenge. Here, we …
expression in order to enhance potency remains a major technical challenge. Here, we …