The chemical synthesis of DNA oligonucleotides and their assembly into synthons, genes,
circuits, and even entire genomes by gene synthesis methods has become an enabling …

DNA assembly is a key part of constructing gene expression systems and even whole
chromosomes. In the past decade, a plethora of powerful new DNA assembly methods …

Recent progress in DNA manipulation and gene circuit engineering has greatly improved
our ability to programme and probe mammalian cell behaviour. These advances have led to …

Crassulacean acid metabolism (CAM) is a specialized mode of photosynthesis that features
nocturnal CO 2 uptake, facilitates increased water‐use efficiency (WUE), and enables CAM …

Synthetic biology seeks to extend approaches from engineering and computation to
redesign of biology, with goals such as generating new chemicals, improving human health …

Engineering mammalian cell lines that stably express many transgenes requires the precise
insertion of large amounts of heterologous DNA into well-characterized genomic loci, but …

Developing predictive models of multi‐protein genetic systems to understand and optimize
their behavior remains a combinatorial challenge, particularly when measurement …

Sequence-specific nucleases such as TALEN and the CRISPR/Cas9 system have so far
been used to disrupt, correct or insert transgenes at precise locations in mammalian …

Human induced pluripotent stem cells (hiPSCs) have potential for personalized and
regenerative medicine. While most of the methods using these cells have focused on …

Despite significant clinical progress in cell and gene therapies, maximizing protein
expression in order to enhance potency remains a major technical challenge. Here, we …