Contrast has traditionally been produced in electron-microscopy of weak phase objects by
simply defocusing the objective lens. There now is renewed interest, however, in using …

Highlights•Phase plate has a long history in transmission electron microscopy (TEM).•Phase
plate could enhance biological TEM image contrast significantly.•Phase plate is useful in …

In this paper, we review the current state of phase plate imaging in a transmission electron
microscope. We focus especially on the hole-free phase plate design, also referred to as the …

Cryo-tomography in the electron microscope is unique in its ability to provide high-
resolution, three-dimensional structural information about cells, organelles and …

Phase plates are a new technique in the field of cryo-electron microscopy. They provide
improved contrast and signal-to-noise ratio in images of radiation sensitive specimens. Thin …

Polymerization of actin into filaments can push membranes forming extensions like filopodia
or lamellipodia, which are important during processes such as cell motility and …

In the recent years, nanotechnology has rapidly evolved as promising toolbox for many
applications, including sensing and drug delivery. Nanotechnology aims at forming man …

Using two levels of electron beam lithography, vapor phase deposition techniques, and FIB
etching, we have fabricated an electrostatic Boersch phase plate for contrast enhancement …

Quantitative measurements by electron microscopy are becoming increasingly important
because we are often concerned with establishing quantitative relationships between the …

We report the implementation of an electrostatic Einzel lens (Boersch) phase plate in a
prototype transmission electron microscope dedicated to aberration-corrected cryo-EM. The …