Synthetic oligonucleotide analogs have greatly aided our understanding of several
biochemical processes. Efficient solid-phase and enzyme-assisted synthetic methods and …

Nearly every DNA polymerase characterized to date exclusively catalyzes the incorporation
of mononucleotides into a growing primer using a DNA or RNA template as a guide to direct …

It is generally assumed that an enzyme-substrate (ES) complex contains all components
necessary for catalysis and that conversion to products occurs by rearrangement of atoms …

Cells throughout nature contain multiple and overlapping DNA repair pathways that are
essential for maintaining the integrity of genomic DNA. 1, 2 The DNA repair pathway known …

Cellular DNA is continually damaged by a plethora of extrinsic and intrinsic sources,
including UV light from the sun and reactive oxygen species resulting from aerobic …

On careful examination of existing kinetic data for correct and incorrect dNTP incorporations
by a variety of DNA polymerases, it is apparent that these enzymes resist a unified …

Pre-steady state kinetics of misincorporation were used to investigate the addition of single
nucleotides to nascent RNA by Escherichia coli RNA polymerase during transcription …

EXCISION of introns from nuclear precursors to messenger RNAs (pre-mRNAs) by the
spliceosome requires two distinct phosphodiester transfer (transesterification) reactions …

We have isolated a large number of polynucleotide kinase ribozymes from a pool of RNA
molecules consisting of an ATP-binding domain flanked by regions of random sequence …

We show that T7 DNA polymerase exists in three distinct structural states, as reported by a
conformationally sensitive fluorophore attached to the recognition (fingers) domain. The …