Comparison of yeasts as hosts for recombinant protein production

AM Vieira Gomes, T Souza Carmo, L Silva Carvalho… - Microorganisms, 2018 - mdpi.com
Recombinant protein production emerged in the early 1980s with the development of
genetic engineering tools, which represented a compelling alternative to protein extraction …

CRISPR-mediated genome editing in non-conventional yeasts for biotechnological applications

P Cai, J Gao, Y Zhou - Microbial cell factories, 2019 - Springer
Non-conventional yeasts are playing important roles as cell factories for bioproduction of
biofuels, food additives and proteins with outstanding natural characteristics. However, the …

Global metabolic rewiring of the nonconventional yeast Ogataea polymorpha for biosynthesis of the sesquiterpenoid β-elemene

M Ye, J Gao, YJ Zhou - Metabolic engineering, 2023 - Elsevier
Bioproduction of natural products via microbial cell factories is a promising alternative to
traditional plant extraction. Recently, nonconventional microorganisms have emerged as …

Bioprospecting Kluyveromyces marxianus as a Robust Host for Industrial Biotechnology

M Bilal, L Ji, Y Xu, S Xu, Y Lin, HMN Iqbal… - … in Bioengineering and …, 2022 - frontiersin.org
Kluyveromyces marxianus is an emerging non-conventional food-grade yeast that is
generally isolated from diverse habitats, like kefir grain, fermented dairy products, sugar …

Bottom-up approaches to engineered living materials: Challenges and future directions

S Molinari, RF Tesoriero, CM Ajo-Franklin - Matter, 2021 - cell.com
Biomaterials made by living systems, while very diverse, generally share similar
organization: different lineages of cells are precisely patterned within a matrix to create a …

Multiplex genome editing of microorganisms using CRISPR-Cas

B Adiego-Perez, P Randazzo, JM Daran… - FEMS microbiology …, 2019 - academic.oup.com
Microbial production of chemical compounds often requires highly engineered microbial cell
factories. During the last years, CRISPR-Cas nucleases have been repurposed as powerful …

Recombination machinery engineering for precise genome editing in methylotrophic yeast Ogataea polymorpha

J Gao, N Gao, X Zhai, YJ Zhou - Iscience, 2021 - cell.com
Methanol biotransformation can expand biorefinery substrate spectrum other than biomass
by using methylotrophic microbes. Ogataea (Hansenula) polymorpha, a representative …

Efficient CRISPR–Cas9 mediated multiplex genome editing in yeasts

L Wang, A Deng, Y Zhang, S Liu, Y Liang, H Bai… - Biotechnology for …, 2018 - Springer
Background The thermotolerant methylotrophic yeast Ogataea polymorpha has been
regarded as an important organism for basic research and biotechnological applications. It …

Biological Parts for Kluyveromyces marxianus Synthetic Biology

AS Rajkumar, JA Varela, H Juergens… - … in bioengineering and …, 2019 - frontiersin.org
Kluyveromyces marxianus is a non-conventional yeast whose physiology and metabolism
lends itself to diverse biotechnological applications. While the wild-type yeast is already in …

Kluyveromyces marxianus: a potential biocatalyst of renewable chemicals and lignocellulosic ethanol production

LV Leonel, PV Arruda, AK Chandel… - Critical Reviews in …, 2021 - Taylor & Francis
Kluyveromyces marxianus is an ascomycetous yeast which has shown promising results in
cellulosic ethanol and renewable chemicals production. It can survive on a variety of carbon …