Serving a robust platform for reverse genetics enabling the in vivo study of gene functions
primarily in enterobacteriaceae, recombineering-or recombination-mediated genetic …

Yeast shuttle vectors are indispensable tools in yeast research. They enable cloning of
defined DNA sequences in Escherichia coli and their direct transfer into Saccharomyces …

All known d-xylose transporters are competitively inhibited by d-glucose, which is one of the
major reasons hampering simultaneous fermentation of d-glucose and d-xylose, two primary …

Background The production of bioethanol from lignocellulose hydrolysates requires a
robust, D-xylose-fermenting and inhibitor-tolerant microorganism as catalyst. The purpose of …

The application of small amounts of natural plant growth hormones, such as gibberellins
(GAs), can increase the productivity and quality of many vegetable and fruit crops. However …

Background The branched chain alcohol isobutanol exhibits superior physicochemical
properties as an alternative biofuel. The yeast Saccharomyces cerevisiae naturally produces …

Background L-tyrosine is a common precursor for a wide range of valuable secondary
metabolites, including benzylisoquinoline alkaloids (BIAs) and many polyketides. An …

Gene inactivation is an essential step in the molecular dissection of gene function. In the
yeast Saccharomyces cerevisiae, many tools for gene disruption are available. Gene …

mRNA localization serves key functions in localized protein production, making it critical that
the translation machinery itself is present at these locations. Here we show that translation …

For thousands of years humans have used the budding yeast Saccharomyces cerevisiae for
the production of bread and alcohol; however, in the last 30–40 years our understanding of …