Rtt105 regulates RPA function by configurationally stapling the flexible domains
Abstract Replication Protein A (RPA) is a heterotrimeric complex that binds to single-
stranded DNA (ssDNA) and recruits over three dozen RPA-interacting proteins to coordinate …
stranded DNA (ssDNA) and recruits over three dozen RPA-interacting proteins to coordinate …
Human HELB is a processive motor protein that catalyzes RPA clearance from single-stranded DNA
S Hormeno, OJ Wilkinson… - Proceedings of the …, 2022 - National Acad Sciences
Human DNA helicase B (HELB) is a poorly characterized helicase suggested to play both
positive and negative regulatory roles in DNA replication and recombination. In this work, we …
positive and negative regulatory roles in DNA replication and recombination. In this work, we …
[HTML][HTML] Rapid long-distance migration of RPA on single stranded DNA occurs through intersegmental transfer utilizing multivalent interactions
Abstract Replication Protein A (RPA) is a single stranded DNA (ssDNA) binding protein that
coordinates diverse DNA metabolic processes including DNA replication, repair, and …
coordinates diverse DNA metabolic processes including DNA replication, repair, and …
An Aurora B-RPA signaling axis secures chromosome segregation fidelity
Errors in chromosome segregation underlie genomic instability associated with cancers.
Resolution of replication and recombination intermediates and protection of vulnerable …
Resolution of replication and recombination intermediates and protection of vulnerable …
Fluorescent human RPA to track assembly dynamics on DNA
DNA metabolic processes including replication, repair, recombination, and telomere
maintenance occur on single-stranded DNA (ssDNA). In each of these complex processes …
maintenance occur on single-stranded DNA (ssDNA). In each of these complex processes …
Long DNA constructs to study helicases and nucleic acid translocases using optical tweezers
C Aicart-Ramos, S Hormeno, OJ Wilkinson… - Methods in …, 2022 - Elsevier
Single molecule biophysics experiments for the study of DNA-protein interactions usually
require production of a homogeneous population of long DNA molecules with controlled …
require production of a homogeneous population of long DNA molecules with controlled …
Generation of site-specifically labelled fluorescent human XPA to investigate DNA binding dynamics during nucleotide excision repair
Nucleotide excision repair (NER) promotes genomic integrity by removing bulky DNA
adducts introduced by external factors such as ultraviolet light. Defects in NER enzymes are …
adducts introduced by external factors such as ultraviolet light. Defects in NER enzymes are …
Site-specific labeling of SBDS to monitor interactions with the 60S ribosomal subunit
Abstract The Shwachman-Diamond syndrome (SDS) is a rare inherited ribosomopathy that
is predominantly caused by mutations in the Shwachman-Bodian-Diamond Syndrome gene …
is predominantly caused by mutations in the Shwachman-Bodian-Diamond Syndrome gene …
Rad52 sorts and stacks Rad51 at the DNA junction to promote homologous recombination
Homologous recombination (HR) repairs double-stranded DNA breaks (DSBs). The DSBs
are resected to yield single-stranded DNA (ssDNA) that are coated by Replication Protein A …
are resected to yield single-stranded DNA (ssDNA) that are coated by Replication Protein A …
Rtt105 configurationally staples RPA and blocks facilitated exchange and interactions with RPA-interacting proteins
Abstract Replication Protein A (RPA) binds to single-stranded DNA (ssDNA) and recruits
over three dozen RPA-interacting proteins (RIPs) to coordinate multiple aspects of DNA …
over three dozen RPA-interacting proteins (RIPs) to coordinate multiple aspects of DNA …