To elucidate principles operating in native biological systems and to develop novel
biotechnologies, synthetic biology aims to build and integrate synthetic gene circuits within …

Within mammalian systems, there exists enormous opportunity to use synthetic gene circuits
to enhance phenotype-based drug discovery, to map the molecular origins of disease, and …

Epigenetic cell memory allows distinct gene expression patterns to persist in different cell
types despite a common genotype. Although different patterns can be maintained by the …

Advances in synthetic biology have provided genetic tools to reprogram cells to obtain a
variety of desired cellular functions. These include tools to enable the customization of cells …

During development, cell state transitions are coordinated through changes in the identity of
molecular regulators in a cell type‐and dose‐specific manner. The ability to rationally …

Precise, scalable, and sustainable control of genetic and cellular activities in mammalian
cells is key to developing precision therapeutics and smart biomanufacturing. Here we …

Expanding the genetic toolbox for prokaryotic synthetic biology is a promising strategy for
enhancing the dynamic range of gene expression and enabling new engineered …

Highlights•Engineering cells as living therapeutics offers enhanced disease
targeting.•Delivering mRNA in vivo offers a platform to advance in vivo cellular …

Synthetic expression cassettes provide the ability to control transgene expression in
experimental animal models through external triggers, enabling the study of gene function …

DNA-based hypoxia biosensors conditionally express a gene of interest when a cell is in a
state of inadequate oxygen supply, which is a feature of several acute and chronic diseases …