Glycosylation is crucial in cellular metabolism and survival. Of interest is the role of N-linked
and O-linked glycans in disease states. Robust analytical methods must be defined to …

Together with proteins and fats, carbohydrates are one of the macronutrients in the human
diet. Digestible carbohydrates, such as starch, starch-based products, sucrose, lactose …

Most methods for the analysis of oligosaccharides from biological sources require a glycan
derivatization step: glycans may be derivatized to introduce a chromophore or fluorophore …

1.1. General Considerations Structural intricacies of carbohydrate molecules and their
propensity to form varied linkages, substitutions, and branching patterns have fascinated …

Sialylated human milk oligosaccharides (SHMOs) are important components of human milk
oligosaccharides. Sialic acids are typically found on the nonreducing end and are known …

Chromatographic stationary phases based on porous graphitic carbon were invented 30
years ago, while columns have been commercially available for 20 years. This special …

The characterization of glycosylation is critical for obtaining a comprehensive view of the
regulation and functions of glycoproteins of interest. Due to the complex nature of …

ANaerobic AMMonium OXidation (anammox) is an established process for efficient nitrogen
removal from wastewater, relying on anammox bacteria to form stable biofilms or granules …

Protein glycosylation is a common post-translational modification that has significant impacts
on protein folding, lifespan, conformation, distribution and function. N-Glycans, which are …

Porous graphitised carbon-liquid chromatography (PGC-LC) has been proven to be a
powerful technique for the analysis and characterisation of complex mixtures of isomeric and …