Optically modulated fluorescence bioimaging: visualizing obscured fluorophores in high background

JC Hsiang, AE Jablonski… - Accounts of chemical …, 2014 - ACS Publications
Conspectus Fluorescence microscopy and detection have become indispensible for
understanding organization and dynamics in biological systems. Novel fluorophores with …

Kinetics of reactive modules adds discriminative dimensions for selective cell imaging

J Quérard, T Le Saux, A Gautier, D Alcor… - …, 2016 - Wiley Online Library
Living cells are chemical mixtures of exceptional interest and significance, whose
investigation requires the development of powerful analytical tools fulfilling the demanding …

Photoswitching kinetics and phase‐sensitive detection add discriminative dimensions for selective fluorescence imaging

J Querard, TZ Markus, MA Plamont… - Angewandte …, 2015 - Wiley Online Library
Non‐invasive separation‐free protocols are attractive for analyzing complex mixtures. To
increase selectivity, an analysis under kinetic control, through exploitation of the …

Comprehensive analytical model for CW laser induced heat in turbid media

H Erkol, F Nouizi, A Luk, MB Unlu, G Gulsen - Optics Express, 2015 - opg.optica.org
In this work, we present a new analytical approach to model continuous wave laser induced
temperature in highly homogeneous turbid media. First, the diffusion equation is used to …

Tailoring cyanine dark states for improved optically modulated fluorescence recovery

DP Mahoney, EA Owens, C Fan… - The Journal of …, 2015 - ACS Publications
Cyanine dyes are well-known for their bright fluorescence and utility in biological imaging.
However, cyanines also readily photoisomerize to produce nonemissive dark states. Co …

[HTML][HTML] Synchronously amplified photoacoustic image recovery (SAPhIRe)

AA Demissie, D VanderLaan, MS Islam, S Emelianov… - Photoacoustics, 2020 - Elsevier
In molecular and cellular photoacoustic imaging with exogenous contrast agents, image
contrast is plagued by background resulting from endogenous absorbers in tissue. By using …

Triplet shelving in fluorescein and its derivatives provides delayed, background-free fluorescence detection

AA Demissie, RM Dickson - The Journal of Physical Chemistry A, 2020 - ACS Publications
Fluorescence from the xanthene dyes rose bengal, erythrosine B, eosin Y, and fluorescein is
modulated by reversibly optically populating and depopulating their long-lived triplet states …

Dark state-modulated fluorescence correlation spectroscopy for quantitative signal recovery

JC Hsiang, BC Fleischer… - The journal of physical …, 2016 - ACS Publications
Excitation of few-atom Ag cluster fluorescence produces significant steady-state dark state
populations that can be dynamically optically depopulated with long wavelength …

Expanding discriminative dimensions for analysis and imaging

J Querard, A Gautier, T Le Saux, L Jullien - Chemical Science, 2015 - pubs.rsc.org
Eliminating the contribution of interfering compounds is a key step in chemical analysis. In
complex media, one possible approach is to perform a preliminary separation. However …

Optically activated delayed fluorescence through control of cyanine dye photophysics

DP Mahoney, AA Demissie… - The Journal of Physical …, 2019 - ACS Publications
Merocyanine 540 fluorescence can be enhanced by optically depopulating dark
photoisomer states to regenerate the fluorescence-generating manifold of the all-trans …