CRISPR/Cas9 genome editing has gained rapidly increasing attentions in recent years,
however, the translation of this biotechnology into therapy has been hindered by efficient …

Electroporation (EP) is a commonly used strategy to increase cell permeability for
intracellular cargo delivery or irreversible cell membrane disruption using electric fields. In …

Plastic particles impact the propagation of antibiotic resistance genes (ARGs) in
environmental media, and their perturbation on the horizontal gene transfer (HGT) of ARGs …

Intracellular delivery of advanced therapeutics, including biologicals and supramolecular
agents, is complex because of the natural biological barriers that have evolved to protect the …

The CRISPR-Cas system initiated a revolution in genome editing when it was, for the first
time, demonstrated success in the mammalian cells. Today, scientists are able to readily edit …

Gene editing is a versatile technique in biomedicine that promotes fundamental research as
well as clinical therapy. The development of Clustered Regularly Interspaced Short …

Intracellular delivery of proteins is receiving considerable attention in biotherapeutics for
various diseases by replacing dysfunctional proteins. Successful intracellular protein …

Cell-penetrating peptides (CPPs) have been discovered to deliver chemical drugs, nucleic
acids, and macromolecules to permeate cell membranes, creating a novel route for …

Gene therapy provides a promising strategy for curing monogenetic disorders and complex
diseases. However, there are challenges associated with the use of viral delivery vectors …

Manipulation of cells for applications such as biomanufacturing and cell-based therapeutics
involves introducing biomolecular cargoes into cells. However, successful delivery is a …