Proteomic analysis of cells, tissues and body fluids has generated valuable insights into the
complex processes influencing human biology. Proteins represent intermediate phenotypes …

Many research questions in fields such as personalized medicine, drug screens or systems
biology depend on obtaining consistent and quantitatively accurate proteomics data from …

This review provides a brief overview of the development of data‐independent acquisition
(DIA) mass spectrometry‐based proteomics and selected DIA data analysis tools. Various …

The data‐independent acquisition mass spectrometry (DIA‐MS) has rapidly evolved as a
powerful alternative for highly reproducible proteome profiling with a unique strength of …

Quantitative phosphoproteomics has transformed investigations of cell signaling, but it
remains challenging to scale the technology for high-throughput analyses. Here we report a …

Data independent acquisition (DIA) is an attractive alternative to standard shotgun
proteomics methods for quantitative experiments. However, most DIA methods require …

Data-independent acquisition approaches typically rely on experiment-specific spectrum
libraries, requiring offline fractionation and tens to hundreds of injections. We demonstrate a …

Protein phosphorylation is a major regulator of protein function and biological outcomes.
This was first recognized through functional biochemical experiments, and in the past …

Human blood plasma provides a highly accessible window to the proteome of any individual
in health and disease. Since its inception in 2002, the Human Proteome Organization's …

To date, the effects of specific modification types and sites on protein lifetime have not been
systematically illustrated. Here, we describe a proteomic method, DeltaSILAC, to …