Genome-scale metabolic models (GEMs) have become a popular tool for systems biology,
and they have been used in many fields such as industrial biotechnology and systems …

Enforcing obligate coupling of growth with synthesis of a desired product has become a key
principle for metabolic engineering of microbial production strains. Various methods from …

Highlights•We briefly review and compare four EFM-based methods for in silico strain
design.•Constrained minimal cut sets and AutoDesign predict deletion targets.•Casop and …

There is currently a strong interest to derive the biological precursor cis, cis-muconic acid
from shikimate pathway-branches to develop a biological replacement for adipic acid …

The modular cell design principle is formulated to devise modular (chassis) cells. These
cells can be assembled with exchangeable production modules in a plug-and-play fashion …

Overproduction of desired native or nonnative biochemical (s) in (micro) organisms can be
achieved through metabolic engineering. Appropriate rewiring of cell metabolism is …

Elementary flux modes (EFMs) are non‐decomposable steady‐state fluxes through
metabolic networks. Every possible flux through a network can be described as a …

Background Production of chemicals from engineered organisms in a batch culture involves
an inherent trade-off between productivity, yield, and titer. Existing strategies for strain …

Background Knockout strategies, particularly the concept of constrained minimal cut sets
(cMCSs), are an important part of the arsenal of tools used in manipulating metabolic …

Background The rational, in silico prediction of gene-knockouts to turn organisms into
efficient cell factories is an essential and computationally challenging task in metabolic …