Computational methods for strain-level microbial detection in colony and metagenome sequencing data

C Anyansi, TJ Straub, AL Manson, AM Earl… - Frontiers in …, 2020 - frontiersin.org
Metagenomic sequencing is a powerful tool for examining the diversity and complexity of
microbial communities. Most widely used tools for taxonomic profiling of metagenomic …

A comparison of tools for the simulation of genomic next-generation sequencing data

M Escalona, S Rocha, D Posada - Nature Reviews Genetics, 2016 - nature.com
Computer simulation of genomic data has become increasingly popular for assessing and
validating biological models or for gaining an understanding of specific data sets. Several …

[HTML][HTML] Design, construction, and in vivo augmentation of a complex gut microbiome

AG Cheng, PY Ho, A Aranda-Díaz, S Jain, BY Feiqiao… - Cell, 2022 - cell.com
Efforts to model the human gut microbiome in mice have led to important insights into the
mechanisms of host-microbe interactions. However, the model communities studied to date …

Healthy human gut phageome

P Manrique, B Bolduc, ST Walk… - Proceedings of the …, 2016 - National Acad Sciences
The role of bacteriophages in influencing the structure and function of the healthy human gut
microbiome is unknown. With few exceptions, previous studies have found a high level of …

Evolutionary dynamics of bacteria in the gut microbiome within and across hosts

NR Garud, BH Good, O Hallatschek, KS Pollard - PLoS biology, 2019 - journals.plos.org
Gut microbiota are shaped by a combination of ecological and evolutionary forces. While the
ecological dynamics have been extensively studied, much less is known about how species …

Single-molecule sequencing and optical mapping yields an improved genome of woodland strawberry (Fragaria vesca) with chromosome-scale contiguity

PP Edger, R VanBuren, M Colle, TJ Poorten… - …, 2018 - academic.oup.com
Background Although draft genomes are available for most agronomically important plant
species, the majority are incomplete, highly fragmented, and often riddled with assembly …

A taxonomic signature of obesity in the microbiome? Getting to the guts of the matter

MM Finucane, TJ Sharpton, TJ Laurent, KS Pollard - PloS one, 2014 - journals.plos.org
Obesity is an important and intractable public health problem. In addition to the well-known
risk factors of behavior, diet, and genetics, gut microbial communities were recently …

Average genome size estimation improves comparative metagenomics and sheds light on the functional ecology of the human microbiome

S Nayfach, KS Pollard - Genome biology, 2015 - Springer
Average genome size is an important, yet often overlooked, property of microbial
communities. We developed MicrobeCensus to rapidly and accurately estimate average …

Daring to be differential: metabarcoding analysis of soil and plant-related microbial communities using amplicon sequence variants and operational taxonomical units

L Joos, S Beirinckx, A Haegeman, J Debode… - BMC genomics, 2020 - Springer
Background Microorganisms are not only indispensable to ecosystem functioning, they are
also keystones for emerging technologies. In the last 15 years, the number of studies on …

Simulating Illumina metagenomic data with InSilicoSeq

H Gourlé, O Karlsson-Lindsjö, J Hayer… - …, 2019 - academic.oup.com
Motivation The accurate in silico simulation of metagenomic datasets is of great importance
for benchmarking bioinformatics tools as well as for experimental design. Users are …