piggyBac Transposony

K Yusa - Mobile DNA III, 2015 - Wiley Online Library
The piggyBac transposon superfamily is a relatively recently recognized transposon
superfamily. The original piggyBac transposon was isolated from the genome of the …

[HTML][HTML] Efficient transposition of the piggyBac (PB) transposon in mammalian cells and mice

S Ding, X Wu, G Li, M Han, Y Zhuang, T Xu - Cell, 2005 - cell.com
Transposable elements have been routinely used for genetic manipulation in lower
organisms, including generating transgenic animals and insertional mutagenesis. In …

Exploring systemic RNA interference in insects: a genome-wide survey for RNAi genes in Tribolium

Y Tomoyasu, SC Miller, S Tomita, M Schoppmeier… - Genome biology, 2008 - Springer
Background RNA interference (RNAi) is a highly conserved cellular mechanism. In some
organisms, such as Caenorhabditis elegans, the RNAi response can be transmitted …

Efficient CRISPR-mediated gene targeting and transgene replacement in the beetle Tribolium castaneum

AF Gilles, JB Schinko, M Averof - Development, 2015 - journals.biologists.com
Gene-editing techniques are revolutionizing the way we conduct genetics in many
organisms. The CRISPR/Cas nuclease has emerged as a highly versatile, efficient and …

Larval RNAi in Tribolium (Coleoptera) for analyzing adult development

Y Tomoyasu, RE Denell - Development genes and evolution, 2004 - Springer
We report here on the use of RNA interference (RNAi) to create pupal and adult loss-of-
function phenotypes in the red flour beetle, Tribolium castaneum, by injection of double …

Gene vector and transposable element behavior in mosquitoes

DA O'Brochta, N Sethuraman… - Journal of …, 2003 - journals.biologists.com
The development of efficient germ-line transformation technologies for mosquitoes has
increased the ability of entomologists to find, isolate and analyze genes. The utility of the …

Enhanced genome assembly and a new official gene set for Tribolium castaneum

N Herndon, J Shelton, L Gerischer, P Ioannidis… - BMC genomics, 2020 - Springer
Background The red flour beetle Tribolium castaneum has emerged as an important model
organism for the study of gene function in development and physiology, for ecological and …

Dissecting Systemic RNA Interference in the Red Flour Beetle Tribolium castaneum: Parameters Affecting the Efficiency of RNAi

SC Miller, K Miyata, SJ Brown, Y Tomoyasu - PloS one, 2012 - journals.plos.org
The phenomenon of RNAi, in which the introduction of dsRNA into a cell triggers the
destruction of the corresponding mRNA resulting in a gene silencing effect, is conserved …

The Tribolium chitin synthase genes TcCHS1 and TcCHS2 are specialized for synthesis of epidermal cuticle and midgut peritrophic matrix

Y Arakane, S Muthukrishnan, KJ Kramer… - Insect molecular …, 2005 - Wiley Online Library
Functional analysis of the two chitin synthase genes, TcCHS1 and TcCHS2, in the red flour
beetle, Tribolium castaneum, revealed unique and complementary roles for each gene …

Functional specialization among insect chitinase family genes revealed by RNA interference

Q Zhu, Y Arakane, RW Beeman… - Proceedings of the …, 2008 - National Acad Sciences
The biological functions of individual members of the large family of chitinase-like proteins
from the red flour beetle, Tribolium castaneum (Tc), were examined by using gene-specific …