Directed enzyme evolution
ET Farinas, T Bulter, FH Arnold - Current opinion in biotechnology, 2001 - Elsevier
Laboratory evolutionists continue to generate better enzymes for industrial and research
applications. Exciting developments include new biocatalysts for enantioselective carbon …
applications. Exciting developments include new biocatalysts for enantioselective carbon …
Differences in amino acids composition and coupling patterns between mesophilic and thermophilic proteins
XX Zhou, YB Wang, YJ Pan, WF Li - Amino acids, 2008 - Springer
Thermophilic proteins show substantially higher intrinsic thermal stability than their
mesophilic counterparts. Amino acid composition is believed to alter the intrinsic stability of …
mesophilic counterparts. Amino acid composition is believed to alter the intrinsic stability of …
Complete genome sequence of the hyperthermophilic archaeon Thermococcus kodakaraensis KOD1 and comparison with Pyrococcus genomes
T Fukui, H Atomi, T Kanai, R Matsumi… - Genome …, 2005 - genome.cshlp.org
The genus Thermococcus, comprised of sulfur-reducing hyperthermophilic archaea,
belongs to the order Thermococcales in Euryarchaeota along with the closely related genus …
belongs to the order Thermococcales in Euryarchaeota along with the closely related genus …
[HTML][HTML] Comparative genomics of Thermus thermophilus and Deinococcus radiodurans: divergent routes of adaptation to thermophily and radiation resistance
MV Omelchenko, YI Wolf, EK Gaidamakova… - BMC evolutionary …, 2005 - Springer
Thermus thermophilus and Deinococcus radiodurans belong to a distinct bacterial clade but
have remarkably different phenotypes. T. thermophilus is a thermophile, which is relatively …
have remarkably different phenotypes. T. thermophilus is a thermophile, which is relatively …
Comparative complete genome sequence analysis of the amino acid replacements responsible for the thermostability of Corynebacterium efficiens
Y Nishio, Y Nakamura, Y Kawarabayasi… - Genome …, 2003 - genome.cshlp.org
Corynebacterium efficiens is the closest relative of Corynebacterium glutamicum, a species
widely used for the industrial production of amino acids. C. efficiens but not C. glutamicum …
widely used for the industrial production of amino acids. C. efficiens but not C. glutamicum …
Identification of thermophilic species by the amino acid compositions deduced from their genomes
DP Kreil, CA Ouzounis - Nucleic acids research, 2001 - academic.oup.com
The global amino acid compositions as deduced from the complete genomic sequences of
six thermophilic archaea, two thermophilic bacteria, 17 mesophilic bacteria and two …
six thermophilic archaea, two thermophilic bacteria, 17 mesophilic bacteria and two …
A snapshot of a transition state analogue of a novel thermophilic esterase belonging to the subfamily of mammalian hormone-sensitive lipase
EST2 is a novel thermophilic carboxylesterase, isolated and cloned from Alicyclobacillus
(formerly Bacillus) acidocaldarius, which optimally hydrolyses esters with acyl chain lengths …
(formerly Bacillus) acidocaldarius, which optimally hydrolyses esters with acyl chain lengths …
Large‐scale prediction of protein geometry and stability changes for arbitrary single point mutations
AJ Bordner, RA Abagyan - Proteins: Structure, Function, and …, 2004 - Wiley Online Library
We have developed a method to both predict the geometry and the relative stability of point
mutants that may be used for arbitrary mutations. The geometry optimization procedure was …
mutants that may be used for arbitrary mutations. The geometry optimization procedure was …
Distinctive gene and protein characteristics of extremely piezophilic Colwellia
Background The deep ocean is characterized by low temperatures, high hydrostatic
pressures, and low concentrations of organic matter. While these conditions likely select for …
pressures, and low concentrations of organic matter. While these conditions likely select for …
A comparison of proteins from Pyrococcus furiosus and Pyrococcus abyssi: barophily in the physicochemical properties of amino acids and in the genetic code
M Di Giulio - Gene, 2005 - Elsevier
A comparison is made between orthologous proteins from a nonbarophile (Pyrococcus
furiosus) and a barophile (Pyrococcus abyssi) organism. The pattern of asymmetries in the …
furiosus) and a barophile (Pyrococcus abyssi) organism. The pattern of asymmetries in the …