High cholesterol/low cholesterol: effects in biological membranes: a review

WK Subczynski, M Pasenkiewicz-Gierula… - Cell biochemistry and …, 2017 - Springer
Lipid composition determines membrane properties, and cholesterol plays a major role in
this determination as it regulates membrane fluidity and permeability, as well as induces the …

[HTML][HTML] Giant unilamellar vesicle electroformation: what to use, what to avoid, and how to quantify the results

Z Boban, I Mardešić, WK Subczynski, M Raguz - Membranes, 2021 - mdpi.com
Since its inception more than thirty years ago, electroformation has become the most
commonly used method for growing giant unilamellar vesicles (GUVs). Although the method …

[HTML][HTML] Membrane models and experiments suitable for studies of the cholesterol bilayer domains

I Mardešić, Z Boban, WK Subczynski, M Raguz - Membranes, 2023 - mdpi.com
Cholesterol (Chol) is an essential component of animal cell membranes and is most
abundant in plasma membranes (PMs) where its concentration typically ranges from 10 to …

[HTML][HTML] Electrostatic switch mechanisms of membrane protein trafficking and regulation

RJ Clarke - Biophysical Reviews, 2023 - Springer
Lipid-protein interactions are normally classified as either specific or general. Specific
interactions refer to lipid binding to specific binding sites within a membrane protein, thereby …

Characterizing the supported lipid membrane formation from cholesterol-rich bicelles

TN Sut, S Park, Y Choe, NJ Cho - Langmuir, 2019 - ACS Publications
Supported lipid bilayers (SLBs) are simplified model membrane systems that mimic the
fundamental properties of biological cell membranes and allow the surface-sensitive tools to …

Formation of cholesterol-rich supported membranes using solvent-assisted lipid self-assembly

SR Tabaei, JA Jackman, SO Kim, B Liedberg, W Knoll… - Langmuir, 2014 - ACS Publications
This paper describes the application of a solvent-exchange method to prepare supported
membranes containing high fractions of cholesterol (up to∼ 57 mol%) in an apparent …

Benzyl isothiocyanate ameliorates high-fat/cholesterol/cholic acid diet-induced nonalcoholic steatohepatitis through inhibiting cholesterol crystal-activated NLRP3 …

HW Chen, CC Yen, LL Kuo, CW Lo, CS Huang… - Toxicology and Applied …, 2020 - Elsevier
Incidence of nonalcoholic fatty liver disease is increasing worldwide. Activation of the
NLRP3 inflammasome is central to the development of diet-induced nonalcoholic …

[HTML][HTML] Electroformation of Giant Unilamellar Vesicles from Damp Lipid Films Formed by Vesicle Fusion

Z Boban, I Mardešić, SP Jozić, J Šumanovac… - Membranes, 2023 - mdpi.com
Giant unilamellar vesicles (GUVs) are artificial membrane models which are of special
interest to researchers because of their similarity in size to eukaryotic cells. The most …

Changes in the properties and organization of human lens lipid membranes occurring with age

L Mainali, M Raguz, WJ O'Brien… - Current eye …, 2017 - Taylor & Francis
Purpose: This research was undertaken to document the changes in the organization and
properties of human lens lipid membranes that occur with age. Methods: Human lens lipid …

[HTML][HTML] Mechanical properties of the high cholesterol-containing membrane: An AFM study

NK Khadka, R Timsina, E Rowe, M O'Dell… - Biochimica et Biophysica …, 2021 - Elsevier
Cholesterol (Chol) content in most cellular membranes does not exceed 50 mol%, only in
the eye lens's fiber cell plasma membrane, its content surpasses 50 mol%. At this high …