Effect of polyethylene glycol on polysaccharides: From molecular modification, composite matrixes, synergetic properties to embeddable application in food fields

L Gong, J Zhu, Y Yang, S Qiao, L Ma, H Wang… - Carbohydrate …, 2024 - Elsevier
Polyethylene glycol (PEG) is a flexible, water-soluble, non-immunogenic, as well as
biocompatible polymer, and it could synergize with polysaccharides for food applications …

Role of Associated Water Dynamics on Protein Stability and Activity in Crowded Milieu

T Khan, B Halder, N Das, P Sen - The Journal of Physical …, 2024 - ACS Publications
Macromolecular crowding bridges in vivo and in vitro studies by simulating cellular
complexities such as high viscosity and limited space while maintaining the experimental …

Macromolecular crowding: how shape and interaction affect the structure, function, conformational dynamics and relative domain movement of a multi-domain protein

N Das, P Sen - Physical Chemistry Chemical Physics, 2022 - pubs.rsc.org
The cellular environment is crowded by macromolecules of various sizes, shapes, and
charges, which modulate protein structure, function and dynamics. Herein, we contemplated …

Shape-dependent macromolecular crowding on the thermodynamics and microsecond conformational dynamics of protein unfolding revealed at the single-molecule …

N Das, P Sen - The Journal of Physical Chemistry B, 2020 - ACS Publications
One of the main differences in the intercellular environment compared to the laboratory
condition is the presence of macromolecular crowders of various compositions, sizes, and …

Associated water dynamics might be a key factor affecting protein stability in the crowded milieu

N Das, E Tarif, A Dutta, P Sen - The Journal of Physical Chemistry …, 2023 - ACS Publications
Over the past 20 years, the most studied and debated aspect of macromolecular crowding is
how it affects protein stability. Traditionally, it is explained by a delicate balance between the …

Boosting the kinetic efficiency of formate dehydrogenase by combining the effects of temperature, high pressure and co-solvent mixtures

MW Jaworek, NF Gajardo-Parra, G Sadowski… - Colloids and Surfaces B …, 2021 - Elsevier
The application of co-solvents and high pressure has been shown to be an efficient means
to modify the kinetics of enzyme-catalyzed reactions without compromising enzyme stability …

Physicochemical characterization of polymer‐stabilized Coacervate protocells

NA Yewdall, BC Buddingh, WJ Altenburg… - …, 2019 - Wiley Online Library
The bottom‐up construction of cell mimics has produced a range of membrane‐bound
protocells that have been endowed with functionality and biochemical processes …

Size-dependent macromolecular crowding effect on the thermodynamics of protein unfolding revealed at the single molecular level

N Das, P Sen - International journal of biological macromolecules, 2019 - Elsevier
The real biological environment involves a high degree of complexity and the
macromolecular crowder is the best candidate to somewhat mimic this. In this contribution …

Application of circular dichroism spectroscopy in studying protein folding, stability, and interaction

MA Haque, P Kaur, A Islam, MI Hassan - Advances in Protein Molecular and …, 2022 - Elsevier
Circular dichroism (CD) is a powerful spectroscopic technique used to study the changes in
the structure and conformation of a protein. The wavelength ranges used for the structural …

Origin of Strong Hydrogen Bonding and Preferred Orientation of Water at Uncharged Polyethylene Glycol Polymer/Water Interface

A Patra, A Bandyopadhyay, S Roy… - The Journal of Physical …, 2023 - ACS Publications
Polyethylene glycol (PEG), a water-soluble non-ionic polymer, finds diverse applications
from Li-ion batteries to drug delivery. The effectiveness of PEG in these contexts hinges on …