Gene drives are selfish genetic elements that are transmitted to progeny at super-Mendelian
(> 50%) frequencies. Recently developed CRISPR–Cas9-based gene-drive systems are …

Mosquito-borne diseases, such as dengue and malaria, pose significant global health
burdens. Unfortunately, current control methods based on insecticides and environmental …

In the human malaria vector Anopheles gambiae, the gene doublesex (Agdsx) encodes two
alternatively spliced transcripts, dsx-female (AgdsxF) and dsx-male (AgdsxM), that control …

Abstract Cas9/gRNA-mediated gene-drive systems have advanced development of genetic
technologies for controlling vector-borne pathogen transmission. These technologies …

CRISPR-based gene-drives targeting the gene doublesex in the malaria vector Anopheles
gambiae effectively suppressed the reproductive capability of mosquito populations reared …

Human pathogens that are transmitted by insects are a global problem, particularly those
vectored by mosquitoes; for example, malaria parasites transmitted by Anopheles species …

Aedes aegypti is the principal mosquito vector for many arboviruses that increasingly infect
millions of people every year. With an escalating burden of infections and the relative failure …

Genetic-based methods offer environmentally friendly species-specific approaches for
control of insect pests. One method, CRISPR homing gene drive that target genes essential …

CRISPR homing gene drives can convert heterozygous cells with one copy of the drive
allele into homozygotes, thereby enabling super-Mendelian inheritance. Such a mechanism …

Homing-based gene drives use a germline source of nuclease to copy themselves at
specific target sites in a genome and bias their inheritance. Such gene drives can be …