Cell-free gene expression (CFE) emerged as an alternative approach to living cells for
specific applications in protein synthesis and labelling for structural biology and proteomics …

E. coli is most preferred system used for the production of recombinant proteins in bacteria
and the availability of improved genetic tools/methods are making it more valuable than …

Escherichia coli is one of the organisms of choice for the production of recombinant proteins.
Its use as a cell factory is well-established and it has become the most popular expression …

Escherichia coli is the most widely used heterologous protein expression system. However,
this system remains a challenge due to the low solubility of proteins, insufficient yield, and …

Highlights•The need for recombinant proteins both as biopharmaceuticals and for structural
studies in drug development is outlined.•The basis of the production of recombinant proteins …

Nanobodies are single-domain antibodies of camelid origin. We generated nanobodies
against the vertebrate nuclear pore complex (NPC) and used them in STORM imaging to …

Recombinant protein expression in Escherichia coli (E. coli) is simple, fast, inexpensive, and
robust, with the expressed protein comprising up to 50 percent of the total cellular protein …

The ability to engineer enzymes and other proteins to any desired stability would have wide-
ranging applications. Here, we demonstrate that computational design of a library with …

Protein function requires the folded protein form, but this form is unstable mainly because it
readily unfolds into a flexible, unstructured form. Protein folding is favored by burying of …

Protein folding is a biophysical process by which proteins reach a specific three-dimensional
structure. The amino acid sequence of a polypeptide chain contains all the information …