Genetic code expansion: inception, development, commercialization

M Manandhar, E Chun… - Journal of the American …, 2021 - ACS Publications
Virtually all natural proteins are built from only 20 amino acids, and while this makes
possible all the functions they perform, the ability to encode other amino acids selected for …

Site-specific incorporation of unnatural amino acids into Escherichia coli recombinant protein: methodology development and recent achievement

S Smolskaya, YA Andreev - Biomolecules, 2019 - mdpi.com
More than two decades ago a general method to genetically encode noncanonical or
unnatural amino acids (NAAs) with diverse physical, chemical, or biological properties in …

Site-specific bioconjugation approaches for enhanced delivery of protein therapeutics and protein drug carriers

RM Lieser, D Yur, MO Sullivan, W Chen - Bioconjugate chemistry, 2020 - ACS Publications
Proteins have the capacity to treat a multitude of diseases both as therapeutics and as drug
carriers due to their complex functional properties, specificity toward binding partners …

Directed-evolution of translation system for efficient unnatural amino acids incorporation and generalizable synthetic auxotroph construction

H Zhao, W Ding, J Zang, Y Yang, C Liu, L Hu… - Nature …, 2021 - nature.com
Site-specific incorporation of unnatural amino acids (UAAs) with similar incorporation
efficiency to that of natural amino acids (NAAs) and low background activity is extremely …

Translation system engineering in Escherichia coli enhances non‐canonical amino acid incorporation into proteins

R Gan, JG Perez, ED Carlson, I Ntai… - Biotechnology and …, 2017 - Wiley Online Library
The ability to site‐specifically incorporate non‐canonical amino acids (ncAAs) into proteins
has made possible the study of protein structure and function in fundamentally new ways, as …

Escherichia coli Extract-Based Cell-Free Expression System as an Alternative for Difficult-to-Obtain Protein Biosynthesis

S Smolskaya, YA Logashina, YA Andreev - International Journal of …, 2020 - mdpi.com
Before utilization in biomedical diagnosis, therapeutic treatment, and biotechnology, the
diverse variety of peptides and proteins must be preliminarily purified and thoroughly …

[HTML][HTML] Exploring protein phosphorylation by combining computational approaches and biochemical methods

G Pérez-Mejías, A Velázquez-Cruz… - Computational and …, 2020 - Elsevier
Post-translational modifications of proteins expand their functional diversity, regulating the
response of cells to a variety of stimuli. Among these modifications, phosphorylation is the …

Genetic incorporation of noncanonical amino acids using two mutually orthogonal quadruplet codons

ED Hankore, L Zhang, Y Chen, K Liu, W Niu… - ACS synthetic …, 2019 - ACS Publications
Genetic incorporation of noncanonical amino acids has emerged as a powerful tool for the
study of protein structure and function. While the three triplet nonsense codons have been …

Reaching New Heights in Genetic Code Manipulation with High Throughput Screening

BR Lino, SJ Williams, ME Castor… - Chemical …, 2024 - ACS Publications
The chemical and physical properties of proteins are limited by the 20 canonical amino
acids. Genetic code manipulation allows for the incorporation of noncanonical amino acids …

Biochemistry of aminoacyl tRNA Synthetase and tRNAs and their engineering for cell-free and synthetic cell applications

RB Ganesh, SJ Maerkl - Frontiers in bioengineering and …, 2022 - frontiersin.org
Cell-free biology is increasingly utilized for engineering biological systems, incorporating
novel functionality, and circumventing many of the complications associated with cells. The …