Is enzyme immobilization a mature discipline? Some critical considerations to capitalize on the benefits of immobilization
JM Bolivar, JM Woodley… - Chemical Society …, 2022 - pubs.rsc.org
Enzyme immobilization has been developing since the 1960s and although many industrial
biocatalytic processes use the technology to improve enzyme performance, still today we …
biocatalytic processes use the technology to improve enzyme performance, still today we …
[HTML][HTML] Stabilization of enzymes via immobilization: Multipoint covalent attachment and other stabilization strategies
RC Rodrigues, Á Berenguer-Murcia… - Biotechnology …, 2021 - Elsevier
The use of enzymes in industrial processes requires the improvement of their features in
many instances. Enzyme immobilization, a requirement to facilitate the recovery and reuse …
many instances. Enzyme immobilization, a requirement to facilitate the recovery and reuse …
Use of Alcalase in the production of bioactive peptides: A review
VG Tacias-Pascacio, R Morellon-Sterling… - International journal of …, 2020 - Elsevier
This review aims to cover the uses of the commercially available protease Alcalase in the
production of biologically active peptides since 2010. Immobilization of Alcalase has also …
production of biologically active peptides since 2010. Immobilization of Alcalase has also …
Parameters necessary to define an immobilized enzyme preparation
Biocatalytic processes continue to find increasing application in industry. Therefore enzyme
immobilization has also become of increasing importance as a means of allowing enzyme …
immobilization has also become of increasing importance as a means of allowing enzyme …
Applications of immobilized lipases in enzymatic reactors: A review
Lipases are efficient biocatalysts with numerous applications in different industrial sectors,
such as pharmaceutical, food, and fine chemistry industries. Enzyme immobilization further …
such as pharmaceutical, food, and fine chemistry industries. Enzyme immobilization further …
[HTML][HTML] Lipase immobilization via cross-linked enzyme aggregates: Problems and prospects–A review
CS Sampaio, JAF Angelotti… - International journal of …, 2022 - Elsevier
In this review we have focused on the preparation of cross-linked enzyme aggregates
(CLEAs) from lipases, as these are among the most used enzyme in bioprocesses. This …
(CLEAs) from lipases, as these are among the most used enzyme in bioprocesses. This …
Immobilization of lipases on hydrophobic supports: immobilization mechanism, advantages, problems, and solutions
RC Rodrigues, JJ Virgen-Ortíz, JCS Dos Santos… - Biotechnology …, 2019 - Elsevier
Lipases are the most widely used enzymes in biocatalysis, and the most utilized method for
enzyme immobilization is using hydrophobic supports at low ionic strength. This method …
enzyme immobilization is using hydrophobic supports at low ionic strength. This method …
Integrating enzyme immobilization and protein engineering: An alternative path for the development of novel and improved industrial biocatalysts
C Bernal, K Rodriguez, R Martínez - Biotechnology Advances, 2018 - Elsevier
Enzyme immobilization often achieves reusable biocatalysts with improved operational
stability and solvent resistance. However, these modifications are generally associated with …
stability and solvent resistance. However, these modifications are generally associated with …
Strategies for the one-step immobilization–purification of enzymes as industrial biocatalysts
In this review, we detail the efforts performed to couple the purification and the
immobilization of industrial enzymes in a single step. The use of antibodies, the …
immobilization of industrial enzymes in a single step. The use of antibodies, the …
Importance of the support properties for immobilization or purification of enzymes
Immobilization and purification of enzymes are usual requirements for their industrial use.
Both purification and immobilization have a common factor: they use a solid activated …
Both purification and immobilization have a common factor: they use a solid activated …