Microbial cyclodextrin glycosyltransferases: Sources, production, and application in cyclodextrin synthesis

K Saini, VM Pathak, A Tyagi, R Gupta - Catalysis Research, 2022 - lidsen.com
Cyclodextrin glycosyltransferase (CGTase) is a multifunctional enzyme that hydrolyzes the α-
glycosidic bond between two sugar molecules and synthesizes cyclodextrins (CDs) and …

Emergence of nanomaterials as potential immobilization supports for whole cell biocatalysts and cell toxicity effects

SA Abdul Manaf, SFZ Mohamad Fuzi… - Biotechnology and …, 2021 - Wiley Online Library
The traditional approach of fermentation by a free cell system has limitations of low
productivity and product separation that need to be addressed for production enhancement …

Efficient secretory expression of Bacillus stearothermophilus α/β-cyclodextrin glycosyltransferase in Bacillus subtilis

L Su, Y Li, J Wu - Journal of Biotechnology, 2021 - Elsevier
Bacillus stearothermophilus α/β-cyclodextrin glycosyltransferase (α/β-CGTase) is an
excellent transglycosylase with broad potential for food application, but its expression level …

Hydrodynamic effects on biofilm development and recombinant protein expression

A Soares, LC Gomes, GA Monteiro, FJ Mergulhão - Microorganisms, 2022 - mdpi.com
Hydrodynamics play an important role in the rate of cell attachment and nutrient and oxygen
transfer, which can affect biofilm development and the level of recombinant protein …

Using concanavalinA as a spacer for immobilization of E. coli onto magnetic nanoparticles

MY Zhuang, C Wang, MQ Xu, XM Ling, JJ Shen… - International Journal of …, 2017 - Elsevier
ConcanavalinA (conA) is a protein extracted from the concanavalin, which has specific
recognition through mannose components on bacterial cell surfaces. A magnetic …

Enhanced excretion of recombinant cyclodextrin glucanotransferase and cell stability of immobilized recombinant Escherichia coli by reducing tryptone concentration

RC Man, NHA Manas, RM Illias… - Journal of Chemical …, 2023 - Wiley Online Library
BACKGROUND Recombinant protein excretion has become a mainstream strategy in
reducing downstream processing costs. However, recombinant protein excretion is often …

Specific Immobilization of Escherichia coli Expressing Recombinant Glycerol Dehydrogenase on Mannose-Functionalized Magnetic Nanoparticles

FL Li, MY Zhuang, JJ Shen, XM Fan, H Choi, JK Lee… - Catalysts, 2018 - mdpi.com
Mannose-functionalized magnetic nanoparticles were prepared for the immobilization of
Escherichia coli cells harboring the recombinant glycerol dehydrogenase gene …

Current advances in obtaining novel cyclodextrin glycosyltransferases for optimizing the synthesis of cyclodextrins

ICB Muniz, JB Santos, RM de OLIVEIRA, FG Santos… - Process …, 2024 - Elsevier
The cyclodextrins (CDs) market has increased due to their numerous applications,
especially in the food and pharmaceutical industries. The high cost of synthetizing one …

Effects of electrospun nanofiber fabrications on immobilization of recombinant Escherichia coli for production of xylitol from glucose

NM Sukri, NHA Manas, NR Jaafar, RA Rahman… - Enzyme and Microbial …, 2024 - Elsevier
A suitable nanofiber sheet was formulated and developed based on its efficacy in the
immobilization of recombinant Escherichia coli (E. coli) to enhance xylitol production. The …

Carbon nanomaterial properties help to enhance xylanase production from recombinant Kluyveromyces lactis through a cell immobilization method

SA Abdul Manaf, SFZ Mohamad Fuzi, KO Low… - Applied Microbiology …, 2021 - Springer
Carbon nanomaterials, due to their catalytic activity and high surface area, have potential as
cell immobilization supports to increase the production of xylanase. Recombinant …