The native chemical ligation reaction (NCL) involves reacting a C-terminal peptide thioester
with an N-terminal cysteinyl peptide to produce a native peptide bond between the two …

Protecting group chemistry for the cysteine thiol group has enabled a vast array of peptide
and protein chemistry over the last several decades. Increasingly sophisticated strategies for …

Protein semisynthesis—defined herein as the assembly of a protein from a combination of
synthetic and recombinant fragments—is a burgeoning field of chemical biology that has …

▪ Abstract In just a few short years, the chemical ligation of unprotected peptide segments in
aqueous solution has established itself as the most practical method for the total synthesis of …

The investigation of biological processes by chemical methods, commonly referred to as
chemical biology, often requires chemical access to biologically relevant macromolecules …

The total chemical synthesis of proteins has great potential for increasing our understanding
of the molecular basis of protein function. The introduction of native chemical ligation …

Although chemists can synthesize virtually any small organic molecule, our ability to
rationally manipulate the structures of proteins is quite limited, despite their involvement in …

▪ Abstract Expressed protein ligation (EPL) is a protein engineering approach that allows
recombinant and synthetic polypeptides to be chemoselectively and regioselectively joined …

Solid-phase peptide synthesis (SPPS) is generally the method of choice for the chemical
synthesis of peptides, allowing routine synthesis of virtually any type of peptide sequence …

The technique of native chemical ligation enables the total chemical synthesis of proteins.
This method is limited, however, by an absolute requirement for a cysteine residue at the …