Extra-column band broadening effects in contemporary liquid chromatography: Causes and solutions

G Desmet, K Broeckhoven - TrAC Trends in Analytical Chemistry, 2019 - Elsevier
Columns for liquid chromatography produce ever narrower peaks and analysts continue to
push to smaller column diameters to save solvents and have better MS responses. The …

[图书][B] Ion-exchange chromatography of proteins

S Yamamoto, K Nakanishi, R Matsuno - 1988 - books.google.com
Showing chemists how to predict ion-exchange chromatography (IEC) separation behavior
and how to determine appropriate operating conditions, this reference illustrates …

Advances and challenges in extremely high-pressure liquid chromatography in current and future analytical scale column formats

K Broeckhoven, G Desmet - Analytical chemistry, 2019 - ACS Publications
The present study speculates on the potential gain we can expect of a further leap in
pressure by moving from the current 1500 bar to a futuristic 3000 bar as well as reviews the …

[图书][B] Encyclopedia of Chromatography 2004 Update Supplement

J Cazes - 2004 - taylorfrancis.com
In step with novel technologies and methodologies that have reshaped chromatography in
recent years, this supplement reviews developments in HPLC, TLC, SFC, CCC, and other …

Advances in ultrahigh-pressure liquid chromatography technology and system design

J De Vos, K Broeckhoven, S Eeltink - Analytical chemistry, 2016 - ACS Publications
The analysis problems encountered in chemical industry and life-science research have
spurred the development of comprehensive solutions to increase the separation efficiency …

Salient sub-second separations

MF Wahab, RM Wimalasinghe, Y Wang… - Analytical …, 2016 - ACS Publications
Sub-second liquid chromatography in very short packed beds is demonstrated as a broad
proof of concept for chiral, achiral, and HILIC separations of biologically important …

Effect of pre-and post-column band broadening on the performance of high-speed chromatography columns under isocratic and gradient conditions

K Vanderlinden, K Broeckhoven… - … of Chromatography A, 2016 - Elsevier
We report on the results of an experimental and theoretical study of the effect of the extra-
column band broadening (ECBB) on the performance of narrow-bore columns filled with the …

Instrumental idiosyncrasies affecting the performance of ultrafast chiral and achiral sub/supercritical fluid chromatography

CL Barhate, MF Wahab, DJ Tognarelli… - Analytical …, 2016 - ACS Publications
It is widely accepted that column technology is ahead of existing chromatographic
instruments. The chromatographic output may not reflect the true picture of the peak profile …

Extracolumn band broadening in capillary liquid chromatography

A Prüß, C Kempter, J Gysler, T Jira - Journal of Chromatography A, 2003 - Elsevier
A commercially available capillary LC instrument was modified to investigate and control the
contribution of different instrument components on extracolumn band broadening …

Measurement and modeling of extra-column effects due to injection and connections in capillary liquid chromatography

JP Grinias, B Bunner, M Gilar, JW Jorgenson - Chromatography, 2015 - mdpi.com
As column volumes continue to decrease, extra-column band broadening has become an
increasingly important consideration when determining column performance. Combined …