Genome editing technologies operate by inducing site-specific DNA perturbations that are
resolved by cellular DNA repair pathways. Products of genome editors include DNA breaks …

Highlights•CRISPR–Cas systems promoted the engineering of non-model bacteria.•Tools
include genome modifications and finely-tuned transcriptional regulation.•CRISPR–Cas …

Whole-genome synthesis provides a powerful approach for understanding and expanding
organism function,–. To build large genomes rapidly, scalably and in parallel, we need (1) …

Engineering the plastid genome based on homologous recombination is well developed in
a few model species. Homologous recombination is also the rule in mitochondria, but …

Microbial cell factories (MCFs) convert low-cost carbon sources into valuable compounds.
The CRISPR/Cas9 system has revolutionized MCF construction as a remarkable genome …

Metabolic engineering holds the promise to transform the chemical industry and to support
the transition into a circular bioeconomy, by engineering cellular biocatalysts that efficiently …

Prime editing (PE) is a precise genome manipulation technology based on the “search and
replace” approach of the CRISPR-Cas9 system, while it does not require the exogenous …

While there are several genome editing techniques available, few are suitable for dynamic
and simultaneous mutagenesis of arbitrary targeted sequences in prokaryotes. Here, to …

Genetically engineered bacteria (GEB) have shown significant promises to revolutionize
modern medicine. These engineered bacteria with unique properties such as enhanced …

Prime editing (PE), as a “search-and-replace” genome editing technology, has shown the
attractive potential of versatile genome editing ability, which is, in principle, currently …