Directed evolution has revolutionized biomolecular engineering by applying cycles of
mutation, amplification and selection to genes of interest (GOIs). However, classical directed …

Precise replacement of an allele with an elite allele controlling an important agronomic trait
in a predefined manner by gene editing technologies is highly desirable in crop …

Gene editing is a powerful tool for genome and cell engineering. Exemplified by CRISPR–
Cas, gene editing could cause DNA damage and trigger DNA repair processes that are …

CRISPR/Cas9 genome-editing tools have tremendously boosted our capability of
manipulating the eukaryotic genomes in biomedical research and innovative …

Retrons are bacterial genetic elements involved in anti-phage defense. They have the
unique ability to reverse transcribe RNA into multicopy single-stranded DNA (msDNA) that …

CRISPR/Cas-based gene-editing technologies have emerged as one of the most
transformative tools in genome science over the past decade, providing unprecedented …

The industrial yeast Komagataella phaffii is a highly effective platform for heterologous
protein production, owing to its high protein expression and secretion capacity …

Hydrogels have emerged as promising biomaterials for nerve regeneration due to their
adjustable properties, structural resemblance to the extracellular matrix, and ability to …

Base editors (BEs) enable efficient, programmable installation of point mutations while
avoiding the use of double-strand breaks. Simultaneous application of two or more different …

Programmable genome insertion (or knock-in) is vital for both fundamental and translational
research. The continuously expanding number of CRISPR-based genome insertion …