Use of chromatographic and electrophoretic tools for assaying elastase, collagenase, hyaluronidase, and tyrosinase activity

S Fayad, P Morin, R Nehmé - Journal of Chromatography A, 2017 - Elsevier
Elastase, collagenase, hyaluronidase and tyrosinase, are very interesting enzymes due to
their direct implication in skin aging and as therapeutic hits. Different techniques can be …

Determination of enzymatic activity by capillary electrophoresis

Z Glatz - Journal of Chromatography B, 2006 - Elsevier
Enzymes are biological catalysts that play an important role in biochemical reactions
necessary for normal growth, maturation and reproduction through whole live world. Their …

MEKC of desmosine and isodesmosine in urine of chronic destructive lung disease patients

S Viglio, P Iadarola, A Lupi, R Trisolini… - European …, 2000 - Eur Respiratory Soc
Degradation of extracellular matrix components is central to many pathological features of
chronic destructive lung disorders. Desmosine and isodesmosine are elastin-derived cross …

[HTML][HTML] A capillary electrophoresis method for the characterization of ecto-nucleoside triphosphate diphosphohydrolases (NTPDases) and the analysis of inhibitors by …

J Iqbal, P Vollmayer, N Braun, H Zimmermann… - Purinergic …, 2005 - Springer
A capillary electrophoresis (CE) method for the characterization of recombinant NTPDases
1, 2, and 3, and for assaying NTPDase inhibitors has been developed performing the …

A highly sensitive CE‐UV method with dynamic coating of silica‐fused capillaries for monitoring of nucleotide pyrophosphatase/phosphodiesterase reactions

J Iqbal, SA Lévesque, J Sévigny, CE Müller - Electrophoresis, 2008 - Wiley Online Library
A new highly sensitive capillary electrophoresis (CE) method applying dynamic coating and
on‐line stacking for the monitoring of nucleotide pyrophosphatases/phosphodiesterases …

Collagenase production in an Antarctic strain of Arthrobotrys tortor Jarowaja

S Tosi, L Annovazzi, I Tosi, P Iadarola, G Caretta - Mycopathologia, 2002 - Springer
This paper describes the results of a comparative screening between the nematophagous
Antarctic fungus Arthrobotrys tortor and other species of that genus for the production of …

Development of off‐line and on‐line capillary electrophoresis methods for the screening and characterization of adenosine kinase inhibitors and substrates

J Iqbal, JC Burbiel, CE Müller - Electrophoresis, 2006 - Wiley Online Library
Fast and convenient CE assays were developed for the screening of adenosine kinase (AK)
inhibitors and substrates. In the first method, the enzymatic reaction was performed in a test …

Assaying human neutrophil elastase activity by capillary zone electrophoresis combined with laser-induced fluorescence

S Fayad, R Nehmé, P Lafite, P Morin - Journal of Chromatography A, 2015 - Elsevier
Skin aging is a progressive process determining the ultimate skin appearance. Human
neutrophil elastase (HNE) has been shown to play an important role in the degradation of …

Microscale separation methods for enzyme kinetics assays

T Křížek, A Kubíčková - Analytical and bioanalytical chemistry, 2012 - Springer
Miniaturization continues to be one of the leading trends in analytical chemistry and one that
brings advantages that can be particularly beneficial in biochemical research. Use of a …

Development and validation of a capillary electrophoresis method for the characterization of herpes simplex virus type 1 (HSV-1) thymidine kinase substrates and …

J Iqbal, L Scapozza, G Folkers, CE Müller - Journal of Chromatography B, 2007 - Elsevier
A fast, convenient capillary electrophoresis (CE) method was developed for monitoring the
enzymatic reaction of herpes simplex virus type 1 thymidine kinase (HSV-1 TK). The reaction …