Metabolic engineering: methodologies and applications

MJ Volk, VG Tran, SI Tan, S Mishra, Z Fatma… - Chemical …, 2022 - ACS Publications
Metabolic engineering aims to improve the production of economically valuable molecules
through the genetic manipulation of microbial metabolism. While the discipline is a little over …

Review of recent developments in GC–MS approaches to metabolomics-based research

DJ Beale, FR Pinu, KA Kouremenos, MM Poojary… - Metabolomics, 2018 - Springer
Background Metabolomics aims to identify the changes in endogenous metabolites of
biological systems in response to intrinsic and extrinsic factors. This is accomplished …

MetaboAnalyst 2.0—a comprehensive server for metabolomic data analysis

J Xia, R Mandal, IV Sinelnikov… - Nucleic acids …, 2012 - academic.oup.com
First released in 2009, MetaboAnalyst (www. metaboanalyst. ca) was a relatively simple web
server designed to facilitate metabolomic data processing and statistical analysis. With …

[HTML][HTML] Web-based inference of biological patterns, functions and pathways from metabolomic data using MetaboAnalyst

J Xia, DS Wishart - Nature protocols, 2011 - nature.com
MetaboAnalyst is an integrated web-based platform for comprehensive analysis of
quantitative metabolomic data. It is designed to be used by biologists (with little or no …

The moderately efficient enzyme: evolutionary and physicochemical trends shaping enzyme parameters

A Bar-Even, E Noor, Y Savir, W Liebermeister… - Biochemistry, 2011 - ACS Publications
The kinetic parameters of enzymes are key to understanding the rate and specificity of most
biological processes. Although specific trends are frequently studied for individual enzymes …

MSEA: a web-based tool to identify biologically meaningful patterns in quantitative metabolomic data

J Xia, DS Wishart - Nucleic acids research, 2010 - academic.oup.com
Gene set enrichment analysis (GSEA) is a widely used technique in transcriptomic data
analysis that uses a database of predefined gene sets to rank lists of genes from microarray …

[HTML][HTML] Analytical platform for metabolome analysis of microbial cells using methyl chloroformate derivatization followed by gas chromatography–mass spectrometry

KF Smart, RBM Aggio, JR Van Houtte… - Nature protocols, 2010 - nature.com
This protocol describes an analytical platform for the analysis of intra-and extracellular
metabolites of microbial cells (yeast, filamentous fungi and bacteria) using gas …

High-throughput, accurate mass metabolome profiling of cellular extracts by flow injection–time-of-flight mass spectrometry

T Fuhrer, D Heer, B Begemann, N Zamboni - Analytical chemistry, 2011 - ACS Publications
Direct injection of samples on high-resolving mass spectrometers is an effective way to
maximize analytical throughput and yet allow analyte discrimination in complex samples by …

Current metabolomics: practical applications

SP Putri, Y Nakayama, F Matsuda, T Uchikata… - Journal of bioscience …, 2013 - Elsevier
The field of metabolomics continues to grow rapidly over the last decade and has been
proven to be a powerful technology in predicting and explaining complex phenotypes in …

Ultrahigh performance liquid chromatography− tandem mass spectrometry method for fast and robust quantification of anionic and aromatic metabolites

JM Buescher, S Moco, U Sauer, N Zamboni - Analytical chemistry, 2010 - ACS Publications
Quantification of metabolites is of pivotal relevance in biology, where it complements more
established omics techniques such as transcriptomics and proteomics. Here, we present a …