Intracellular delivery by membrane disruption: mechanisms, strategies, and concepts

MP Stewart, R Langer, KF Jensen - Chemical reviews, 2018 - ACS Publications
Intracellular delivery is a key step in biological research and has enabled decades of
biomedical discoveries. It is also becoming increasingly important in industrial and medical …

A review on electroporation-based intracellular delivery

J Shi, Y Ma, J Zhu, Y Chen, Y Sun, Y Yao, Z Yang, J Xie - Molecules, 2018 - mdpi.com
Intracellular delivery is a critical step in biological discoveries and has been widely utilized
in biomedical research. A variety of molecular tools have been developed for cell-based …

Recent advances in microscale electroporation

SE Choi, H Khoo, SC Hur - Chemical reviews, 2022 - ACS Publications
Electroporation (EP) is a commonly used strategy to increase cell permeability for
intracellular cargo delivery or irreversible cell membrane disruption using electric fields. In …

Multiplexed high-throughput localized electroporation workflow with deep learning–based analysis for cell engineering

CA Patino, N Pathak, P Mukherjee, SH Park, G Bao… - Science …, 2022 - science.org
Manipulation of cells for applications such as biomanufacturing and cell-based therapeutics
involves introducing biomolecular cargoes into cells. However, successful delivery is a …

Force-controlled manipulation of single cells: from AFM to FluidFM

O Guillaume-Gentil, E Potthoff, D Ossola… - Trends in …, 2014 - cell.com
The ability to perturb individual cells and to obtain information at the single-cell level is of
central importance for addressing numerous biological questions. Atomic force microscopy …

Microfluidic and nanofluidic intracellular delivery

J Hur, AJ Chung - Advanced Science, 2021 - Wiley Online Library
Innate cell function can be artificially engineered and reprogrammed by introducing
biomolecules, such as DNAs, RNAs, plasmid DNAs, proteins, or nanomaterials, into the …

A comprehensive review on intracellular delivery

D Morshedi Rad, M Alsadat Rad… - Advanced …, 2021 - Wiley Online Library
Intracellular delivery is considered an indispensable process for various studies, ranging
from medical applications (cell‐based therapy) to fundamental (genome‐editing) and …

Microfluidic cell stretching for highly effective gene delivery into hard-to-transfect primary cells

J Hur, I Park, KM Lim, J Doh, SG Cho, AJ Chung - ACS nano, 2020 - ACS Publications
Cell therapy and cellular engineering begin with internalizing synthetic biomolecules and
functional nanomaterials into primary cells. Conventionally, electroporation, lipofection, or …

Universal intracellular biomolecule delivery with precise dosage control

Y Cao, H Chen, R Qiu, M Hanna, E Ma, M Hjort… - Science …, 2018 - science.org
Intracellular delivery of mRNA, DNA, and other large macromolecules into cells plays an
essential role in an array of biological research and clinical therapies. However, current …

Combined numerical and experimental investigation of localized electroporation-based cell transfection and sampling

P Mukherjee, SSP Nathamgari, JA Kessler… - ACS …, 2018 - ACS Publications
Localized electroporation has evolved as an effective technology for the delivery of foreign
molecules into cells while preserving their viability. Consequently, this technique has …