Intracellular delivery by membrane disruption: mechanisms, strategies, and concepts
Intracellular delivery is a key step in biological research and has enabled decades of
biomedical discoveries. It is also becoming increasingly important in industrial and medical …
biomedical discoveries. It is also becoming increasingly important in industrial and medical …
A review on electroporation-based intracellular delivery
Intracellular delivery is a critical step in biological discoveries and has been widely utilized
in biomedical research. A variety of molecular tools have been developed for cell-based …
in biomedical research. A variety of molecular tools have been developed for cell-based …
Recent advances in microscale electroporation
Electroporation (EP) is a commonly used strategy to increase cell permeability for
intracellular cargo delivery or irreversible cell membrane disruption using electric fields. In …
intracellular cargo delivery or irreversible cell membrane disruption using electric fields. In …
Multiplexed high-throughput localized electroporation workflow with deep learning–based analysis for cell engineering
Manipulation of cells for applications such as biomanufacturing and cell-based therapeutics
involves introducing biomolecular cargoes into cells. However, successful delivery is a …
involves introducing biomolecular cargoes into cells. However, successful delivery is a …
Force-controlled manipulation of single cells: from AFM to FluidFM
O Guillaume-Gentil, E Potthoff, D Ossola… - Trends in …, 2014 - cell.com
The ability to perturb individual cells and to obtain information at the single-cell level is of
central importance for addressing numerous biological questions. Atomic force microscopy …
central importance for addressing numerous biological questions. Atomic force microscopy …
Microfluidic and nanofluidic intracellular delivery
Innate cell function can be artificially engineered and reprogrammed by introducing
biomolecules, such as DNAs, RNAs, plasmid DNAs, proteins, or nanomaterials, into the …
biomolecules, such as DNAs, RNAs, plasmid DNAs, proteins, or nanomaterials, into the …
A comprehensive review on intracellular delivery
D Morshedi Rad, M Alsadat Rad… - Advanced …, 2021 - Wiley Online Library
Intracellular delivery is considered an indispensable process for various studies, ranging
from medical applications (cell‐based therapy) to fundamental (genome‐editing) and …
from medical applications (cell‐based therapy) to fundamental (genome‐editing) and …
Microfluidic cell stretching for highly effective gene delivery into hard-to-transfect primary cells
Cell therapy and cellular engineering begin with internalizing synthetic biomolecules and
functional nanomaterials into primary cells. Conventionally, electroporation, lipofection, or …
functional nanomaterials into primary cells. Conventionally, electroporation, lipofection, or …
Universal intracellular biomolecule delivery with precise dosage control
Intracellular delivery of mRNA, DNA, and other large macromolecules into cells plays an
essential role in an array of biological research and clinical therapies. However, current …
essential role in an array of biological research and clinical therapies. However, current …
Combined numerical and experimental investigation of localized electroporation-based cell transfection and sampling
Localized electroporation has evolved as an effective technology for the delivery of foreign
molecules into cells while preserving their viability. Consequently, this technique has …
molecules into cells while preserving their viability. Consequently, this technique has …