Macromolecular crowding and confinement: biochemical, biophysical, and potential physiological consequences
Expected and observed effects of volume exclusion on the free energy of rigid and flexible
macromolecules in crowded and confined systems, and consequent effects of crowding and …
macromolecules in crowded and confined systems, and consequent effects of crowding and …
Weak chemical interactions that drive protein evolution: crowding, sticking, and quinary structure in folding and function
D Guin, M Gruebele - Chemical reviews, 2019 - ACS Publications
In recent years, better instrumentation and greater computing power have enabled the
imaging of elusive biomolecule dynamics in cells, driving many advances in understanding …
imaging of elusive biomolecule dynamics in cells, driving many advances in understanding …
Diffusion, crowding & protein stability in a dynamic molecular model of the bacterial cytoplasm
SR McGuffee, AH Elcock - PLoS computational biology, 2010 - journals.plos.org
A longstanding question in molecular biology is the extent to which the behavior of
macromolecules observed in vitro accurately reflects their behavior in vivo. A number of …
macromolecules observed in vitro accurately reflects their behavior in vivo. A number of …
Evolution of mammalian diving capacity traced by myoglobin net surface charge
Introduction Evolution of extended breath-hold endurance enables the exploitation of the
aquatic niche by numerous mammalian lineages and is accomplished by elevated body …
aquatic niche by numerous mammalian lineages and is accomplished by elevated body …
Analysis of the size dependence of macromolecular crowding shows that smaller is better
KA Sharp - Proceedings of the National Academy of …, 2015 - National Acad Sciences
The aqueous milieu inside cells contains as much as 30–40% dissolved protein and RNA by
volume. This large concentration of macromolecules is expected to cause significant …
volume. This large concentration of macromolecules is expected to cause significant …
Toward an understanding of biochemical equilibria within living cells
Four types of environmental effects that can affect macromolecular reactions in a living cell
are defined: nonspecific intermolecular interactions, side reactions, partitioning between …
are defined: nonspecific intermolecular interactions, side reactions, partitioning between …
Forced degradation studies of biopharmaceuticals: Selection of stress conditions
Stability studies under stress conditions or forced degradation studies play an important role
in different phases of development and production of biopharmaceuticals and biological …
in different phases of development and production of biopharmaceuticals and biological …
[HTML][HTML] Formation of protein complexes in crowded environments–from in vitro to in vivo
Y Phillip, G Schreiber - FEBS letters, 2013 - Elsevier
Traditionally, biochemical studies are performed in dilute homogenous solutions, which are
very different from the dense mixture of molecules found in cells. Thus, the physiological …
very different from the dense mixture of molecules found in cells. Thus, the physiological …
Attractive protein-polymer interactions markedly alter the effect of macromolecular crowding on protein association equilibria
M Jiao, HT Li, J Chen, AP Minton, Y Liang - Biophysical journal, 2010 - cell.com
The dependence of the fluorescence of catalase upon the concentration of added
superoxide dismutase (SOD) indicates that SOD binds to saturable sites on catalase. The …
superoxide dismutase (SOD) indicates that SOD binds to saturable sites on catalase. The …
Protein-complex stability in cells and in vitro under crowded conditions
SS Stadmiller, GJ Pielak - Current Opinion in Structural Biology, 2021 - Elsevier
Highlights•Both steric repulsions and chemical interactions are important.•Systematic
experiments involving temperature, crowder molecular weight and concentration are …
experiments involving temperature, crowder molecular weight and concentration are …