Recent progress in multienzymes co-immobilization and multienzyme system applications

S Ren, C Li, X Jiao, S Jia, Y Jiang, M Bilal… - Chemical Engineering …, 2019 - Elsevier
Enzyme catalysis has been attracting increasing interest in the past twenty years.
Nevertheless, most reports concerning enzyme catalysis have been carried out using single …

[HTML][HTML] Lipase immobilization via cross-linked enzyme aggregates: Problems and prospects–A review

CS Sampaio, JAF Angelotti… - International journal of …, 2022 - Elsevier
In this review we have focused on the preparation of cross-linked enzyme aggregates
(CLEAs) from lipases, as these are among the most used enzyme in bioprocesses. This …

Multienzymatic cascade reactions via enzyme complex by immobilization

ET Hwang, S Lee - ACS Catalysis, 2019 - ACS Publications
Multienzymatic cascade reactions are a most important technology to succeed in industrial
process development, such as synthesis of pharmaceutical, cosmetic, and nutritional …

Lipase promiscuity and its biochemical applications

M Kapoor, MN Gupta - Process Biochemistry, 2012 - Elsevier
Lipases are the most widely used class of enzymes in organic synthesis. Availability of large
number of commercial preparations, their broad specificity and relatively better stability (as …

From protein engineering to immobilization: promising strategies for the upgrade of industrial enzymes

RK Singh, MK Tiwari, R Singh, JK Lee - International journal of molecular …, 2013 - mdpi.com
Enzymes found in nature have been exploited in industry due to their inherent catalytic
properties in complex chemical processes under mild experimental and environmental …

Characteristic features and biotechnological applications of cross-linked enzyme aggregates (CLEAs)

RA Sheldon - Applied microbiology and biotechnology, 2011 - Springer
Cross-linked enzyme aggregates (CLEAs) have many economic and environmental benefits
in the context of industrial biocatalysis. They are easily prepared from crude enzyme …

Perspective of recent progress in immobilization of enzymes

DN Tran, KJ Balkus Jr - Acs Catalysis, 2011 - ACS Publications
The commercial application of biocatalysts depends on the development of effective
methods of immobilization. The immobilization of enzymes greatly increases the stability of …

Cross-linked enzyme aggregates as industrial biocatalysts

RA Sheldon - Organic Process Research & Development, 2011 - ACS Publications
The immobilization of enzymes as cross-linked enzyme aggregates (CLEAs) and their
applications in industrially relevant biotransformations are reviewed. The preparation of …

CLEAs, combi-CLEAs and 'smart'magnetic CLEAs: Biocatalysis in a bio-based economy

RA Sheldon - Catalysts, 2019 - mdpi.com
Biocatalysis has emerged in the last decade as a pre-eminent technology for enabling the
envisaged transition to a more sustainable bio-based economy. For industrial viability it is …

Optimization protocols and improved strategies of cross-linked enzyme aggregates technology: current development and future challenges

JD Cui, SR Jia - Critical reviews in biotechnology, 2015 - Taylor & Francis
Cross-linked enzyme aggregate (CLEA) technology has been regarded as an effective
carrier-free immobilization method. This method is very attractive due to its simplicity and …