Recent advances in discovery, heterologous expression, and molecular engineering of cyclodextrin glycosyltransferase for versatile applications
Cyclodextrin glycosyltransferase (CGTase) is an important enzyme with multiple functions, in
particular the production of cyclodextrins. It is also widely applied in baking and …
particular the production of cyclodextrins. It is also widely applied in baking and …
Computation-aided engineering of starch-debranching pullulanase from Bacillus thermoleovorans for enhanced thermostability
J Bi, S Chen, X Zhao, Y Nie, Y Xu - Applied Microbiology and …, 2020 - Springer
Pullulanases are widely used in food, medicine, and other industries because they
specifically hydrolyze α-1, 6-glycosidic linkages in starch and oligosaccharides. In addition …
specifically hydrolyze α-1, 6-glycosidic linkages in starch and oligosaccharides. In addition …
[HTML][HTML] Metagenomics reveal the role of microorganism and GH genes contribute to Sichuan South-road dark tea quality formation during pile fermentation
Y Zou, Y Yuan, M Liu, X Li, Y Lai, X Liu, L Tan, Q Tang… - LWT, 2023 - Elsevier
Microbes are critical for the flavor development of Sichuan South-road Dark Tea (SSDT)
during pile-fermentation. However, their mechanism of action has not been fully elucidated …
during pile-fermentation. However, their mechanism of action has not been fully elucidated …
Glycosidic bond specificity of glucansucrases: on the role of acceptor substrate binding residues
H Leemhuis, T Pijning, JM Dobruchowska… - Biocatalysis and …, 2012 - Taylor & Francis
Many lactic acid bacteria produce extracellular α-glucan polysaccharides using a
glucansucrase and sucrose as glucose donor. The structure and the physicochemical …
glucansucrase and sucrose as glucose donor. The structure and the physicochemical …
Ligand-binding specificity and promiscuity of the main lignocellulolytic enzyme families as revealed by active-site architecture analysis
L Tian, S Liu, S Wang, L Wang - Scientific reports, 2016 - nature.com
Biomass can be converted into sugars by a series of lignocellulolytic enzymes, which belong
to the glycoside hydrolase (GH) families summarized in CAZy databases. Here, using a …
to the glycoside hydrolase (GH) families summarized in CAZy databases. Here, using a …
Crystal structure of the branching enzyme I (BEI) from Oryza sativa L with implications for catalysis and substrate binding
J Noguchi, K Chaen, NT Vu, T Akasaka… - …, 2011 - academic.oup.com
Starch-branching enzyme catalyzes the cleavage of α-1, 4-linkages and the subsequent
transfer of α-1, 4 glucan to form an α-1, 6 branch point in amylopectin. Sequence analysis of …
transfer of α-1, 4 glucan to form an α-1, 6 branch point in amylopectin. Sequence analysis of …
Gene cloning and enzymatic characterization of alkali‐tolerant type I pullulanase from Exiguobacterium acetylicum
Y Qiao, Q Peng, J Yan, H Wang… - Letters in applied …, 2015 - academic.oup.com
A pullulanase gene (Pul3YH5) of 2568 bp, which encodes a protein containing 855 amino
acid residues, was cloned from the alkaliphilic bacterium Exiguobacterium acetylicum YH5 …
acid residues, was cloned from the alkaliphilic bacterium Exiguobacterium acetylicum YH5 …
Crystal structure of Anoxybacillus α-amylase provides insights into maltose binding of a new glycosyl hydrolase subclass
A new subfamily of glycosyl hydrolase family GH13 was recently proposed for α-amylases
from Anoxybacillus species (ASKA and ADTA), Geobacillus thermoleovorans (GTA, Pizzo …
from Anoxybacillus species (ASKA and ADTA), Geobacillus thermoleovorans (GTA, Pizzo …
Diversity of microbial carbohydrate-active enZYmes (CAZYmes) associated with freshwater and soil samples from Caatinga biome
AC Andrade, A Fróes, FÁC Lopes, FL Thompson… - Microbial ecology, 2017 - Springer
Semi-arid and arid areas occupy about 33% of terrestrial ecosystems. However, little
information is available about microbial diversity in the semi-arid Caatinga, which represents …
information is available about microbial diversity in the semi-arid Caatinga, which represents …
Oligosaccharide and substrate binding in the starch debranching enzyme barley limit dextrinase
Complete hydrolytic degradation of starch requires hydrolysis of both the α-1, 4-and α-1, 6-
glucosidic bonds in amylopectin. Limit dextrinase (LD) is the only endogenous barley …
glucosidic bonds in amylopectin. Limit dextrinase (LD) is the only endogenous barley …