The 26S proteasome is the most complex ATP-dependent protease machinery, of~ 2.5 MDa
mass, ubiquitously found in all eukaryotes. It selectively degrades ubiquitin-conjugated …

We present a perspective of our view of the application of cryoelectron microscopy (cryo-EM)
to structure-based drug design (SBDD). We discuss the basic needs and requirements for …

The proteasome is an ATP-dependent, 2.5-megadalton molecular machine that is
responsible for selective protein degradation in eukaryotic cells. Here we present cryo …

Adenosine triphosphatases (ATPases) associated with a variety of cellular activities (AAA+),
the hexameric ring-shaped motor complexes located in all ATP-driven proteolytic machines …

The proteasome is a well-identified therapeutic target for cancer treatment. It acts as the
main protein degradation system in the cell and degrades key mediators of cell growth …

Protein homeostasis is the basis of normal life activities, and the proteasome family plays an
extremely important function in this process. The proteasome 20S is a concentric circle …

Proline-and arginine-rich peptide PR11 is an allosteric inhibitor of 20S proteasome. We
modified its sequence inter alia by introducing HbYX, RYX, or RHbX C-terminal extensions …

The fundamental importance of the 26S proteasome in health and disease suggests that its
function must be finely controlled, and yet our knowledge about proteasome regulation …

While proteins populating their native conformations constitute the functional entities of cells,
protein aggregates are traditionally associated with cellular dysfunction, stress and disease …

Since the report of “DNA untwisting” activity in 1972,∼ 50 years of research has revealed
seven topoisomerases in humans (TOP1, TOP1mt, TOP2α, TOP2β, TOP3α, TOP3β and …