Extracellular vesicle analysis

A Hendrix, L Lippens, C Pinheiro, C Théry… - Nature Reviews …, 2023 - nature.com
Cells release small, phospholipid membrane-enclosed particles, collectively referred to as
extracellular vesicles (EVs), into their surroundings to enable intercellular communication …

A compendium of single extracellular vesicle flow cytometry

JA Welsh, GJA Arkesteijn, M Bremer… - Journal of …, 2023 - Wiley Online Library
Flow cytometry (FCM) offers a multiparametric technology capable of characterizing single
extracellular vesicles (EVs). However, most flow cytometers are designed to detect cells …

Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches

JA Welsh, DCI Goberdhan, L O'Driscoll… - Journal of …, 2024 - Wiley Online Library
Extracellular vesicles (EVs), through their complex cargo, can reflect the state of their cell of
origin and change the functions and phenotypes of other cells. These features indicate …

Extracellular vesicle measurements with nanoparticle tracking analysis–An accuracy and repeatability comparison between NanoSight NS300 and ZetaView

D Bachurski, M Schuldner, PH Nguyen… - Journal of …, 2019 - Taylor & Francis
The expanding field of extracellular vesicle (EV) research needs reproducible and accurate
methods to characterize single EVs. Nanoparticle Tracking Analysis (NTA) is commonly …

Methodological guidelines to study extracellular vesicles

FAW Coumans, AR Brisson, EI Buzas… - Circulation …, 2017 - Am Heart Assoc
Owing to the relationship between extracellular vesicles (EVs) and physiological and
pathological conditions, the interest in EVs is exponentially growing. EVs hold high hopes …

Therapeutic and diagnostic translation of extracellular vesicles in cardiovascular diseases: roadmap to the clinic

S Sahoo, M Adamiak, P Mathiyalagan, F Kenneweg… - Circulation, 2021 - Am Heart Assoc
Exosomes are small membrane-bound vesicles of endocytic origin that are actively
secreted. The potential of exosomes as effective communicators of biological signaling in …

Precision size and refractive index analysis of weakly scattering nanoparticles in polydispersions

AD Kashkanova, M Blessing, A Gemeinhardt… - Nature …, 2022 - nature.com
Abstract Characterization of the size and material properties of particles in liquid
suspensions is in very high demand, for example, in the analysis of colloidal samples or of …

Extracellular vesicle quantification and characterization: common methods and emerging approaches

TA Hartjes, S Mytnyk, GW Jenster, V van Steijn… - Bioengineering, 2019 - mdpi.com
Extracellular vesicles (EVs) are a family of small membrane vesicles that carry information
about cells by which they are secreted. Growing interest in the role of EVs in intercellular …

Characterization of extracellular vesicles and synthetic nanoparticles with four orthogonal single‐particle analysis platforms

T Arab, ER Mallick, Y Huang, L Dong… - Journal of …, 2021 - Wiley Online Library
We compared four orthogonal technologies for sizing, counting, and phenotyping of
extracellular vesicles (EVs) and synthetic particles. The platforms were: single‐particle …

Size and concentration analyses of extracellular vesicles by nanoparticle tracking analysis: a variation study

B Vestad, A Llorente, A Neurauter… - Journal of …, 2017 - Taylor & Francis
Current methods for characterisation of extracellular vesicles (EVs) need further
standardisation in order to obtain an acceptable level of data comparability. Size and …