Microbial proteases: ubiquitous enzymes with innumerable uses

P Solanki, C Putatunda, A Kumar, R Bhatia, A Walia - 3 Biotech, 2021 - Springer
Proteases are ubiquitous enzymes, having significant physiological roles in both synthesis
and degradation. The use of microbial proteases in food fermentation is an age-old process …

Metagenomics: novel enzymes from non-culturable microbes

F Berini, C Casciello, GL Marcone… - FEMS microbiology …, 2017 - academic.oup.com
In the transition to the post-petroleum economy, there is a growing demand for novel
enzymes with high process performances to replace traditional chemistry with a more …

Metagenomic applications in exploration and development of novel enzymes from nature: a review

FA Prayogo, A Budiharjo, HP Kusumaningrum… - Journal of Genetic …, 2020 - Springer
Background Microbial community has an essential role in various fields, especially the
industrial sector. Microbes produce metabolites in the form of enzymes, which are one of the …

Enzyme immobilization: polymer–solvent–enzyme compatibility

F Asaduzzaman, S Salmon - Molecular Systems Design & …, 2022 - pubs.rsc.org
Immobilization improves enzyme stability, allows easy enzyme separation from reaction
mixtures, and enables repeatable use over prolonged periods, especially in systems …

Cloning, Expression, and Structural Elucidation of a Biotechnologically Potential Alkaline Serine Protease From a Newly Isolated Haloalkaliphilic Bacillus lehensis JO …

HB Bhatt, SP Singh - Frontiers in microbiology, 2020 - frontiersin.org
An alkaline protease gene of Bacillus lehensis JO-26 from saline desert, Little Rann of
Kutch, was cloned and expressed in Escherichia coli BL21 (DE3). A 1,014-bp ORF encoded …

Simultaneous hydrolysis of various protein-rich industrial wastes by a naturally evolved protease from tannery wastewater microbiota

S Ariaeenejad, K Kavousi, ASA Mamaghani… - Science of The Total …, 2022 - Elsevier
Elimination of protein-rich waste materials is one of the vital environmental protection
requirements. Using of non-naturally occurring chemicals for their remediation properties …

A computational learning paradigm to targeted discovery of biocatalysts from metagenomic data: A case study of lipase identification

MF Shahraki, FF Atanaki, S Ariaeenejad… - Biotechnology and …, 2022 - Wiley Online Library
The growing adoption of enzymes as biocatalysts in various industries has accentuated the
demand for acquiring access to the great natural diversity and, in the meantime, the advent …

Trends in extracellular serine proteases of bacteria as detergent bioadditive: alternate and environmental friendly tool for detergent industry

R Salwan, V Sharma - Archives of microbiology, 2019 - Springer
Proteases, one of the largest groups of industrial enzymes occupy a major share in
detergent industry. To meet the existing demands, proteases with efficient catalytic …

A brief account on enzyme mining using metagenomic approach

T Patel, HG Chaudhari, V Prajapati, S Patel… - Frontiers in Systems …, 2022 - frontiersin.org
Metagenomics is an approach for directly analyzing the genomes of microbial communities
in the environment. The use of metagenomics to investigate novel enzymes is critical …

Homology modeling and heterologous expression of highly alkaline subtilisin-like serine protease from Bacillus halodurans C-125

A Tekin, U Uzuner, K Sezen - Biotechnology Letters, 2021 - Springer
Here we report heterologous expression, enzymatic characterization and structure
homology modeling of a subtilisin-like alkaline serine protease (ASP) from Bacillus …