Leaf infiltration in plant science: old method, new possibilities

IA Chincinska - Plant Methods, 2021 - Springer
The penetration of substances from the surface to deep inside plant tissues is called
infiltration. Although various plant tissues may be effectively saturated with externally …

Transient expression systems for plant-derived biopharmaceuticals

TV Komarova, S Baschieri, M Donini… - Expert review of …, 2010 - Taylor & Francis
In the molecular farming area, transient expression approaches for pharmaceutical proteins
production, mainly recombinant monoclonal antibodies and vaccines, were developed …

A novel transcription factor CmMYB012 inhibits flavone and anthocyanin biosynthesis in response to high temperatures in chrysanthemum

LJ Zhou, Z Geng, Y Wang, Y Wang, S Liu… - Horticulture …, 2021 - academic.oup.com
Flavones are among the major colorless pigments synthesized through branches of the
flavonoid pathway in plants. However, due to the absence of a gene encoding flavone …

Transcription factor CmbHLH16 regulates petal anthocyanin homeostasis under different lights in Chrysanthemum

LJ Zhou, Y Wang, Y Wang, A Song, J Jiang… - Plant …, 2022 - academic.oup.com
Light is essential to plant survival and elicits a wide range of plant developmental and
physiological responses under different light conditions. A low red-to-far red (R/FR) light …

CmBBX8 accelerates flowering by targeting CmFTL1 directly in summer chrysanthemum

L Wang, J Sun, L Ren, M Zhou, X Han… - Plant Biotechnology …, 2020 - Wiley Online Library
For a flowering plant, the transition from vegetative stage to reproductive growth is probably
the most critical developmental switch. In the model plant Arabidopsis thaliana, the product …

Optimization and utilization of Agrobacterium-mediated transient protein production in Nicotiana

M Shamloul, J Trusa, V Mett, V Yusibov - JoVE (Journal of Visualized …, 2014 - jove.com
Agrobacterium-mediated transient protein production in plants is a promising approach to
produce vaccine antigens and therapeutic proteins within a short period of time. However …

Predictive models for transient protein expression in tobacco (Nicotiana tabacum L.) can optimize process time, yield, and downstream costs

JF Buyel, R Fischer - Biotechnology and bioengineering, 2012 - Wiley Online Library
The transient expression of recombinant biopharmaceutical proteins in plants can suffer
inter‐batch variation, which is considered a major drawback under the strict regulatory …

Simple plant‐based production and purification of the assembled human ferritin heavy chain as a nanocarrier for tumor‐targeted drug delivery and bioimaging in …

M Knödler, P Opdensteinen… - Biotechnology and …, 2023 - Wiley Online Library
Nanoparticles are used as carriers for the delivery of drugs and imaging agents. Proteins are
safer than synthetic nanocarriers due to their greater biocompatibility and the absence of …

Development of fast and portable frequency magnetic mixing-based serological SARS-CoV-2-specific antibody detection assay

J Pietschmann, N Voepel, L Voß, S Rasche… - Frontiers in …, 2021 - frontiersin.org
A novel severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) emerged in China
in December 2019, causing an ongoing, rapidly spreading global pandemic. Worldwide …

Towards a seamless product and process development workflow for recombinant proteins produced by plant molecular farming

JF Buyel - Biotechnology Advances, 2024 - Elsevier
Plant molecular farming (PMF) has been promoted as a fast, efficient and cost-effective
alternative to bacteria and animal cells for the production of biopharmaceutical proteins …