CRISPR technology: A decade of genome editing is only the beginning
JY Wang, JA Doudna - Science, 2023 - science.org
The advent of clustered regularly interspaced short palindromic repeat (CRISPR) genome
editing, coupled with advances in computing and imaging capabilities, has initiated a new …
editing, coupled with advances in computing and imaging capabilities, has initiated a new …
Microbiome and human health: current understanding, engineering, and enabling technologies
The human microbiome is composed of a collection of dynamic microbial communities that
inhabit various anatomical locations in the body. Accordingly, the coevolution of the …
inhabit various anatomical locations in the body. Accordingly, the coevolution of the …
[HTML][HTML] Engineering the next generation of cell-based therapeutics
Cell-based therapeutics are an emerging modality with the potential to treat many currently
intractable diseases through uniquely powerful modes of action. Despite notable recent …
intractable diseases through uniquely powerful modes of action. Despite notable recent …
Drag-and-drop genome insertion of large sequences without double-strand DNA cleavage using CRISPR-directed integrases
MTN Yarnall, EI Ioannidi, C Schmitt-Ulms… - Nature …, 2023 - nature.com
Programmable genome integration of large, diverse DNA cargo without DNA repair of
exposed DNA double-strand breaks remains an unsolved challenge in genome editing. We …
exposed DNA double-strand breaks remains an unsolved challenge in genome editing. We …
[HTML][HTML] Assessing and advancing the safety of CRISPR-Cas tools: from DNA to RNA editing
CRISPR-Cas gene editing has revolutionized experimental molecular biology over the past
decade and holds great promise for the treatment of human genetic diseases. Here we …
decade and holds great promise for the treatment of human genetic diseases. Here we …
[HTML][HTML] The CRISPR-Cas toolbox and gene editing technologies
G Liu, Q Lin, S Jin, C Gao - Molecular cell, 2022 - cell.com
The emergence of CRISPR-Cas systems has accelerated the development of gene editing
technologies, which are widely used in the life sciences. To improve the performance of …
technologies, which are widely used in the life sciences. To improve the performance of …
[HTML][HTML] Genome engineering for crop improvement and future agriculture
C Gao - Cell, 2021 - cell.com
Feeding the ever-growing population is a major challenge, especially in light of rapidly
changing climate conditions. Genome editing is set to revolutionize plant breeding and …
changing climate conditions. Genome editing is set to revolutionize plant breeding and …
The widespread IS200/IS605 transposon family encodes diverse programmable RNA-guided endonucleases
IscB proteins are putative nucleases encoded in a distinct family of IS200/IS605 transposons
and are likely ancestors of the RNA-guided endonuclease Cas9, but the functions of IscB …
and are likely ancestors of the RNA-guided endonuclease Cas9, but the functions of IscB …
Structural biology of CRISPR–Cas immunity and genome editing enzymes
CRISPR–Cas systems provide resistance against foreign mobile genetic elements and have
a wide range of genome editing and biotechnological applications. In this Review, we …
a wide range of genome editing and biotechnological applications. In this Review, we …
Species-and site-specific genome editing in complex bacterial communities
Understanding microbial gene functions relies on the application of experimental genetics in
cultured microorganisms. However, the vast majority of bacteria and archaea remain …
cultured microorganisms. However, the vast majority of bacteria and archaea remain …