Cryopreservation techniques for ram sperm

A Saha, M Asaduzzaman… - Veterinary medicine …, 2022 - Wiley Online Library
Germplasm storage and transportation in artificial insemination (AI) and other advanced
technologies are facilitated by cryopreservation. In reproduction, the cryopreservation of …

Implications of cryopreservation on structural and functional attributes of bovine spermatozoa: An overview

VR Upadhyay, V Ramesh, RK Dewry, G Kumar… - Andrologia, 2021 - Wiley Online Library
Sperm cryopreservation is an important adjunct to assisted reproduction techniques (ART)
for improving the reproductive efficiency of dairy cattle and buffaloes. Improved …

Antioxidative effects of melatonin on kinetics, microscopic and oxidative parameters of cryopreserved bull spermatozoa

I Ashrafi, H Kohram, FF Ardabili - Animal Reproduction Science, 2013 - Elsevier
Reactive oxygen species generated during the freeze–thawing process may reduce sperm
quality. This study evaluates the effects of melatonin supplementation as an antioxidant in …

[HTML][HTML] Substitution of egg yolk with different concentrations of soybean lecithin in tris-based extender during bulls' semen preservability

A Gamal, WS El-Nattat, RI El-Sheshtawy… - Asian Pacific Journal of …, 2016 - Elsevier
Objective To investigate the effect of various concentrations of soybean lecithin as an
alternative for egg yolk in bull semen extender on post-chilling and post-thaw sperm quality …

Cryopreservation of dog epididymal spermatozoa by conventional freezing or ultra-rapid freezing with nonpermeable cryoprotectant

DA Galarza, G Landi, E Mejía, JX Samaniego… - Cryobiology, 2021 - Elsevier
This study was aimed to assess the effectiveness of two methods for cryopreservation of dog
epididymal spermatozoa, one by conventional freezing (CF) with shortening both …

[HTML][HTML] Two-step accelerating freezing protocol yields a better motility, membranes and DNA integrities of thawed ram sperm than three-steps freezing protocols

DA Galarza, A López-Sebastián, H Woelders… - Cryobiology, 2019 - Elsevier
The present study compares a protocol that mimics freezing of ram semen in static nitrogen
vapor with two protocols with an initial low cooling rate in the first step, followed by higher …

Effect of freezing rate on goat sperm morphology and DNA integrity

B ÜSTÜNER, Z Nur, S Alcay… - Turkish Journal of …, 2015 - journals.tubitak.gov.tr
This study investigates the effect of freezing rates on the spermatological parameters of
frozen and thawed Saanen goat semen. Equilibrated semen was frozen at 4 different …

[HTML][HTML] Anti-oxidative effects of ethanol extract of Origanum vulgare on kinetics, microscopic and oxidative parameters of cryopreserved Holstein bull spermatozoa

H Daghigh Kia, R Farhadi, I Ashrafi… - Iranian Journal of …, 2016 - journals.iau.ir
Origanum vulgare contains high levels of phenolic compounds such as gallic acid and
polyphenols such as rosmarinic acid and quercetin. The purpose of this study was to …

Association between different soy lecithin-based extenders and freezing rates in ram semen cryopreservation

JCJ Molina, MB de Oliveira, PHC Costa… - Animal Reproduction …, 2023 - Elsevier
The aim of the study was to evaluate the effect of the association between glycine-milk (GM)
based extenders made with different concentrations of soy lecithin (SL) and freezing rates …

Optimizing the freezing rate for ovine semen cryopreservation: Phospholipid profiles and functions of the plasma membrane and quality and fertilization of …

Y Fang, H Blair, R Zhong, H Sun, D Zhou - Small Ruminant Research, 2016 - Elsevier
The objective of this study was to investigate the effects of freezing rate on plasma
membrane (PM) phospholipids and the function and fertilization of ram spermatozoa during …