Advances in promoter engineering: novel applications and predefined transcriptional control

AP Cazier, J Blazeck - Biotechnology Journal, 2021 - Wiley Online Library
Synthetic biology continues to progress by relying on more robust tools for transcriptional
control, of which promoters are the most fundamental component. Numerous studies have …

[HTML][HTML] Merging automation and fundamental discovery into the design–build–test–learn cycle of nontraditional microbes

N Gurdo, DC Volke, PI Nikel - Trends in Biotechnology, 2022 - cell.com
Major advances toward a bio-based industry enabled cost-efficient bioproduction of multiple
chemicals, yet successful industrial processes are relatively scarce and limited to the use of …

[HTML][HTML] Mechanisms and biotechnological applications of transcription factors

H He, M Yang, S Li, G Zhang, Z Ding, L Zhang… - Synthetic and systems …, 2023 - Elsevier
Transcription factors play an indispensable role in maintaining cellular viability and finely
regulating complex internal metabolic networks. These crucial bioactive functions rely on …

High-throughput screening for efficient microbial biotechnology

A Sarnaik, A Liu, D Nielsen, AM Varman - Current opinion in biotechnology, 2020 - Elsevier
Highlights•High-throughput screening (HTS) facilitates selection of the best performing
strain.•Biosensor-enabled HTS obviates the need for sophisticated analytical …

[HTML][HTML] Vibrio sp. dhg as a platform for the biorefinery of brown macroalgae

HG Lim, DH Kwak, S Park, S Woo, JS Yang… - Nature …, 2019 - nature.com
Although brown macroalgae holds potential as an alternative feedstock, its utilization by
conventional microbial platforms has been limited due to the inability to metabolize one of …

Genetic circuit-assisted smart microbial engineering

C Gao, P Xu, C Ye, X Chen, L Liu - Trends in microbiology, 2019 - cell.com
Rapid advances in DNA synthesis, genetic manipulation, and biosensors have greatly
improved the ability to engineer microorganisms with complex functions. By accurately …

Multilayer genetic circuits for dynamic regulation of metabolic pathways

S Cui, X Lv, X Xu, T Chen, H Zhang, Y Liu… - ACS Synthetic …, 2021 - ACS Publications
The dynamic regulation of metabolic pathways is based on changes in external signals and
endogenous changes in gene expression levels and has extensive applications in the field …

Sustainable production and biomedical application of polymalic acid from renewable biomass and food processing wastes

X Zou, S Li, P Wang, B Li, Y Feng… - Critical reviews in …, 2021 - Taylor & Francis
Polymalic acid (PMA), a homopolymer of L-malic acid (MA) generated from a yeast-like
fungus Aureobasidium pullulans, has unique properties and many applications in food …

Microbial cell engineering to improve cellular synthetic capacity

Q Ding, W Diao, C Gao, X Chen, L Liu - Biotechnology Advances, 2020 - Elsevier
Rapid technological progress in gene assembly, biosensors, and genetic circuits has led to
reinforce the cellular synthetic capacity for chemical production. However, overcoming the …

Structure‐switching RNAs: from gene expression regulation to small molecule detection

C Husser, N Dentz, M Ryckelynck - Small Structures, 2021 - Wiley Online Library
RNA is instrumental to cell life in many aspects, especially gene expression regulation.
Among the various known regulatory RNAs, riboswitches are particularly interesting cis …