Escherichia coli expression system continues to dominate the bacterial expression systems
and remain to be the preferred system for laboratory investigations and initial development …

Solubility of recombinant proteins (ie, the extent of soluble versus insoluble expression in
heterogeneous hosts) is the first checkpoint criterion for determining recombinant protein …

Precise prediction of prokaryotic translation efficiency can provide valuable information for
optimizing bacterial host for the production of biochemical compounds or recombinant …

Developing predictive models of multi‐protein genetic systems to understand and optimize
their behavior remains a combinatorial challenge, particularly when measurement …

Unnatural amino acid (UAA) incorporation by amber codon suppression offers scientists a
powerful tool to modify the properties of proteins at will. UAA incorporation has been used …

By enabling the simultaneous engagement of two distinct targets, bispecific antibodies
broaden the potential utility of antibody-based therapies. However, bispecific-antibody …

RNA folding plays an important role in controlling protein synthesis as well as other cellular
processes. Existing models have focused on how RNA folding energetics control translation …

Protein expression in Escherichia coli represents the most facile approach for the
preparation of non-glycosylated proteins for analytical and preparative purposes. So far, the …

Current methods for producing immunoglobulin G (IgG) antibodies in engineered cells often
require refolding steps or secretion across one or more biological membranes. Here, we …

We describe protein synthesis, folding and assembly of antibody fragments and full-length
aglycosylated antibodies using an Escherichia coli-based open cell-free synthesis (OCFS) …