Synthetic biology: bottom-up assembly of molecular systems

S Hirschi, TR Ward, WP Meier, DJ Müller… - Chemical …, 2022 - ACS Publications
The bottom-up assembly of biological and chemical components opens exciting
opportunities to engineer artificial vesicular systems for applications with previously unmet …

Polymer–lipid hybrid materials

YK Go, C Leal - Chemical reviews, 2021 - ACS Publications
Hierarchic self-assembly underpins much of the form and function seen in synthetic or
biological soft materials. Lipids are paramount examples, building themselves in nature or …

[HTML][HTML] Proton gradients from light-harvesting E. coli control DNA assemblies for synthetic cells

K Jahnke, N Ritzmann, J Fichtler, A Nitschke… - Nature …, 2021 - nature.com
Bottom-up and top-down approaches to synthetic biology each employ distinct
methodologies with the common aim to harness living systems. Here, we realize a strategic …

Encapsulation of cannabidiol in oil-in-water nanoemulsions and nanoemulsion-filled hydrogels: a structure and biological assessment study

S Demisli, E Galani, M Goulielmaki, FL Kyrilis… - Journal of Colloid and …, 2023 - Elsevier
Hypothesis Lipophilic cannabidiol can be solubilized in oil-in water nanoemulsions, which
can then be impregnated into chitosan hydrogels forming another colloidal system that will …

Polymeric Giant Unilamellar Vesicles with Integrated DNA‐Origami Nanopores: An Efficient Platform for Tuning Bioreaction Dynamics Through Controlled Molecular …

R Cochereau, V Maffeis, EC Dos Santos… - Advanced Functional …, 2023 - Wiley Online Library
Giant unilamellar vesicles (GUVs) are microcompartments serving to confine reactions,
allow signaling pathways, or design synthetic cells. Polymer GUVs are composed of …

Interface engineering in multiphase systems toward synthetic cells and organelles: From soft matter fundamentals to biomedical applications

Z Liu, W Zhou, C Qi, T Kong - Advanced Materials, 2020 - Wiley Online Library
Synthetic cells have a major role in gaining insight into the complex biological processes of
living cells; they also give rise to a range of emerging applications from gene delivery to …

Bottom-up synthesis of artificial cells: recent highlights and future challenges

I Ivanov, SL Castellanos, S Balasbas III… - Annual Review of …, 2021 - annualreviews.org
The bottom-up approach in synthetic biology aims to create molecular ensembles that
reproduce the organization and functions of living organisms and strives to integrate them in …

Increased efficiency of charge-mediated fusion in polymer/lipid hybrid membranes

N Marušič, L Otrin, J Rauchhaus… - Proceedings of the …, 2022 - National Acad Sciences
Due to their augmented properties, biomimetic polymer/lipid hybrid compartments are a
promising substitute for natural liposomes in multiple applications, but the protein-free fusion …

[HTML][HTML] En route to dynamic life processes by SNARE-mediated fusion of polymer and hybrid membranes

L Otrin, A Witkowska, N Marušič, Z Zhao, RB Lira… - Nature …, 2021 - nature.com
A variety of artificial cells springs from the functionalization of liposomes with proteins.
However, these models suffer from low durability without repair and replenishment …

Current problems and future avenues in proteoliposome research

AM Amati, S Graf, S Deutschmann… - Biochemical Society …, 2020 - portlandpress.com
Membrane proteins (MPs) are the gatekeepers between different biological compartments
separated by lipid bilayers. Being receptors, channels, transporters, or primary pumps, they …