Life in Multi-Extreme Environments: Brines, Osmotic and Hydrostatic Pressure─ A Physicochemical View

JM Knop, S Mukherjee, MW Jaworek… - Chemical …, 2022 - ACS Publications
Elucidating the details of the formation, stability, interactions, and reactivity of biomolecular
systems under extreme environmental conditions, including high salt concentrations in …

Effect of high hydrostatic pressure processing on the structure, functionality, and nutritional properties of food proteins: A review

W Wang, P Yang, L Rao, L Zhao, X Wu… - … Reviews in Food …, 2022 - Wiley Online Library
Proteins are important food ingredients that possess both functional and nutritional
properties. High hydrostatic pressure (HHP) is an emerging nonthermal food processing …

Not always sticky: Specificity of protein stabilization by sugars is conferred by protein–water hydrogen bonds

GI Olgenblum, N Carmon, D Harries - Journal of the American …, 2023 - ACS Publications
Solutes added to buffered solutions directly impact protein folding. Protein stabilization by
cosolutes or crowders has been shown to be largely driven by protein–cosolute volume …

Temperature, hydrostatic pressure, and osmolyte effects on liquid–liquid phase separation in protein condensates: physical chemistry and biological implications

H Cinar, Z Fetahaj, S Cinar, RM Vernon… - … A European Journal, 2019 - Wiley Online Library
Liquid–liquid phase separation (LLPS) of proteins and other biomolecules play a critical role
in the organization of extracellular materials and membrane‐less compartmentalization of …

Protecting Proteins from Desiccation Stress Using Molecular Glasses and Gels

GI Olgenblum, BO Hutcheson, GJ Pielak… - Chemical …, 2024 - ACS Publications
Faced with desiccation stress, many organisms deploy strategies to maintain the integrity of
their cellular components. Amorphous glassy media composed of small molecular solutes or …

Crowders and cosolvents—major contributors to the cellular milieu and efficient means to counteract environmental stresses

M Gao, C Held, S Patra, L Arns, G Sadowski… - …, 2017 - Wiley Online Library
The free energy and conformational landscape of biomolecular systems as well as
biochemical reactions depend not only on temperature and pressure, but also on the …

Boosting the kinetic efficiency of formate dehydrogenase by combining the effects of temperature, high pressure and co-solvent mixtures

MW Jaworek, NF Gajardo-Parra, G Sadowski… - Colloids and Surfaces B …, 2021 - Elsevier
The application of co-solvents and high pressure has been shown to be an efficient means
to modify the kinetics of enzyme-catalyzed reactions without compromising enzyme stability …

Reversible morphological control of tubulin-encapsulating giant liposomes by hydrostatic pressure

M Hayashi, M Nishiyama, Y Kazayama, T Toyota… - Langmuir, 2016 - ACS Publications
Liposomes encapsulating cytoskeletons have drawn much recent attention to develop an
artificial cell-like chemical-machinery; however, as far as we know, there has been no report …

Impact of macromolecular crowding and compression on protein–protein interactions and liquid–liquid phase separation phenomena

K Julius, J Weine, M Gao, J Latarius, M Elbers… - …, 2019 - ACS Publications
We determined the intermolecular interaction potential, V (r), of dense lysozyme solutions,
which governs the spatial distribution of the protein molecules and the location of its liquid …

Hydrostatic pressure increases the catalytic activity of amyloid fibril enzymes

TQ Luong, N Erwin, M Neumann… - Angewandte Chemie …, 2016 - Wiley Online Library
We studied the combined effects of pressure (0.1–200 MPa) and temperature (22, 30, and
38° C) on the catalytic activity of designed amyloid fibrils using a high‐pressure stopped …