Directed evolution of stereo‐, regio‐, and chemoselective enzymes constitutes a unique way
to generate biocatalysts for synthetically interesting transformations in organic chemistry and …

The term B-factor, sometimes called the Debye–Waller factor, temperature factor, or atomic
displacement parameter, is used in protein crystallography to describe the attenuation of X …

This review covers the recent developments (since 2015) of enantioselective
desymmetrization reactions using metal-catalyzed, organocatalyzed and enzymatic …

Steric hindrance modification-based pocket reshaping is an effective approach for enzyme–
substrate acceptance evolution. However, previous studies were limited to using the …

The computational study of enantioselective reactions is a challenging task that requires
high accuracy, as very small energy differences have to be reproduced. Quantum chemical …

定向进化通过建立突变体文库与高通量筛选方法, 快速提升蛋白的特定性质,
是目前蛋白质工程最为常用的蛋白质设计改造策略. 近十年随着计算机运算能力大幅提升以及 …

Diaryl ketones are important building blocks for synthesizing pharmaceuticals and are
generally regarded as “difficult-to-reduce” ketones due to the large steric hindrance of their …

The FRISM strategy has emerged as one of the most efficient methods for protein
engineering. It involves the identification of hotspots, which is usually rationalized through …

The versatile synthetic intermediate (2 S, 4 R)-4-hydroxylysine can be produced using l-
lysine hydroxylase. However, the wild-type enzyme cannot effectively catalyze the C4 …

Deep eutectic solvents (DESs), heralded for their synthesis simplicity, economic viability,
and reduced volatility and flammability, have found increasing application in biocatalysis …