This review presents an overview of the dynamically developing field of mass spectrometry‐
based metabolomics. Metabolomics aims at the comprehensive and quantitative analysis of …

Robustness, the ability to maintain performance in the face of perturbations and uncertainty,
is a long-recognized key property of living systems. Owing to intimate links to cellular …

CO2 sequestration engineering is an attractive strategy for achieving carbon-and energy-
efficient bioproduction. However, the efficiency of heterotrophic CO2 sequestration is limited …

Absolute metabolite concentrations are critical to a quantitative understanding of cellular
metabolism, as concentrations impact both the free energies and rates of metabolic …

The soil bacterium Pseudomonas putida KT2440 lacks a functional Embden-Meyerhof-
Parnas (EMP) pathway, and glycolysis is known to proceed almost exclusively through the …

Although the network topology of metabolism is well known, understanding the principles
that govern the distribution of fluxes through metabolism lags behind. Experimentally, these …

Stable isotope, and in particular 13C-based flux analysis, is the exclusive approach to
experimentally quantify the integrated responses of metabolic networks. Here we describe a …

Pentose phosphate pathway and isocitrate dehydrogenase are generally considered to be
the major sources of the anabolic reductant NADPH. As one of very few microbes …

Many properties of complex networks cannot be understood from monitoring the
components—not even when comprehensively monitoring all protein or metabolite …

Gas chromatography–mass spectrometry based metabolite profiling of biological samples is
rapidly becoming one of the cornerstones of functional genomics and systems biology. Thus …